Diagnosis of Acute Toxoplasmosis by an Enzyme Immunoassay for Specific Immunoglobulin M Antibodies

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1983 Jun 1

PMID 6874915

Citations 16

Authors

F Wielaard

H van Gruijthuijsen

W Duermeyer

A W Joss

L Skinner

H Williams

E H van Elven

Affiliations

Soon will be listed here.

Abstract

A recently developed enzyme-linked immunosorbent assay for detection of immunoglobulin M (IgM) class antibodies to Toxoplasma gondii was evaluated with respect to specificity and sensitivity. By using an antibody capture principle and F(ab')2 conjugates, interference of rheumatoid factors was absent. No cross-reactions with anti-toxoplasma IgG occurred, and no interference with antinuclear antibodies was found. A large-scale study with about 1,500 clinical specimens revealed a 100% specificity. By testing 79 sera from patients with acute-phase acquired toxoplasmosis, sensitivity was found to be 97%. In routine clinical practice, the IgM-enzyme-linked immunosorbent assay proved to be a more sensitive tool for diagnosis than the immunofluorescent-antibody test. The course of IgM-enzyme-linked immunosorbent assay antibodies in acute patients was studied; IgM reached peak levels within 1 month after onset of illness, and could be demonstrated up to an average of 8 months after onset.

Citing Articles

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Diagnosis of toxoplasmosis by joint detection of immunoglobulin A and immunoglobulin M.

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Prevalence of latent toxoplasmosis and serological diagnosis of active infection in HIV-positive patients.

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Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection.

Partanen P, Turunen H, Paasivuo R, Leinikki P J Clin Microbiol. 1984; 20(1):133-5.

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