Diagnosis of Toxoplasmosis by Joint Detection of Immunoglobulin A and Immunoglobulin M

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Jun 1

PMID 9163460

Citations 2

Authors

M Arcavi

G Orfus

G Griemberg

Affiliations

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Abstract

An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the results obtained were compared with those obtained by methods routinely used for the detection of acute-phase markers, IgA and IgM IIF and enzyme immunoassay. Accordingly, 114 serum samples were selected from patients showing titers of > or = 1:1,024 by IgG,A,M-IIF. (i) In 90 of the samples, neither IgA nor IgM was detected by any of the methods employed; (ii) the remaining 24 samples showed IgA and/or IgM. In all cases, the IgG,A,M-IIF assay with absorbed sera was positive. These comparative data support the use of IgG,A,M-IIF, performed with absorbed and unabsorbed sera simultaneously, for determining the presence of specific IgG, IgA, and IgM by employing a single technique (IIF), one conjugate (anti-IgG,A,M), and only one sample (with and without previous absorption), thus providing a useful initial tool for the diagnosis of toxoplasmosis.

Citing Articles

Multiplexed Anti-Toxoplasma IgG, IgM, and IgA Assay on Plasmonic Gold Chips: towards Making Mass Screening Possible with Dye Test Precision.

Li X, Pomares C, Gonfrier G, Koh B, Zhu S, Gong M J Clin Microbiol. 2016; 54(7):1726-1733.

PMID: 27008879 PMC: 4922109. DOI: 10.1128/JCM.03371-15.

Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii rop2 protein.

Martin V, Arcavi M, Santillan G, Amendoeira M, De Souza Neves E, Griemberg G Clin Diagn Lab Immunol. 1998; 5(5):627-31.

PMID: 9729528 PMC: 95632. DOI: 10.1128/CDLI.5.5.627-631.1998.

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