Tissue Localization of Esterase-5 in Drosophila Pseudoobscura

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1983 Jun 1

PMID 6870773

Citations 1

Authors

A J Lunday

J L FARMER

Affiliations

Soon will be listed here.

Abstract

Disc gel electrophoresis of dissected adults of Drosophila pseudoobscura showed that most of the esterase-5 activity was in the head (36%) and thorax (51%), with little activity in the abdomen (13%). No activity was found in digestive tissues, reproductive tissues, or nervous tissues. Most of the est-5 activity (61%) was found to be associated with hemolymph. However, the eyes contained 39% of the total est-5 activity. These results were supported by spectrophotometric assays of esterase activity in crude extracts of eyes and whole flies without eyes which showed that 27% of the total est-5 activity was in the eyes.

Citing Articles

Molecular analysis of evolutionary changes in the expression of Drosophila esterases.

Brady J, Richmond R Proc Natl Acad Sci U S A. 1990; 87(21):8217-21.

PMID: 1700427 PMC: 54926. DOI: 10.1073/pnas.87.21.8217.

References

Cook B, FORGASH A . THE IDENTIFICATION AND DISTRIBUTION OF THE CARBOXYLIC ESTERASES IN THE AMERICAN COCKROACH, PERIPLANTETA AMERICANA L. J Insect Physiol. 1965; 11:237-50. DOI: 10.1016/0022-1910(65)90071-5. View

Johnson G . Enzyme polymorphism and metabolism. Science. 1974; 184(4132):28-37. DOI: 10.1126/science.184.4132.28. View

Pasteur N, Kastritsis C . Developmental studies in Drosophila. I. Acid phosphatases, esterases, and other proteins in organs and whole-fly homogenates during development of D. pseudoobscura. Dev Biol. 1971; 26(4):525-36. DOI: 10.1016/0012-1606(71)90138-2. View

Cochrane B, Richmond R . Studies of esterase-6 in Drosophila melanogaster. II. The genetics and frequency distributions of naturally occurring variants studied by electrophoretic and heat stability criteria. Genetics. 1979; 93(2):461-78. PMC: 1214093. DOI: 10.1093/genetics/93.2.461. View

Hubby J, Lewontin R . A molecular approach to the study of genic heterozygosity in natural populations. I. The number of alleles at different loci in Drosophila pseudoobscura. Genetics. 1966; 54(2):577-94. PMC: 1211185. DOI: 10.1093/genetics/54.2.577. View

Berger E, Canter R . The esterases of Drosophila. I. The anodal esterases and their possible role in eclosion. Dev Biol. 1973; 33(1):48-55. DOI: 10.1016/0012-1606(73)90163-2. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

Lewontin R, Hubby J . A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics. 1966; 54(2):595-609. PMC: 1211186. DOI: 10.1093/genetics/54.2.595. View

Prakash S, Lewontin R, Hubby J . A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central, marginal and isolated populations of Drosophila pseudoobscura. Genetics. 1969; 61(4):841-58. PMC: 1212247. DOI: 10.1093/genetics/61.4.841. View

10.

Johnson F, Bealle S . Isozyme variability in species of the genus Drosophila. V. Ejaculatory bulb esterases in Drosophila phylogeny. Biochem Genet. 1968; 2(1):1-18. DOI: 10.1007/BF01458447. View

11.

Yamazaki T . Measurement of fitness at the esterase-5 locus in Drosophila pseudoobscura. Genetics. 1971; 67(4):579-603. PMC: 1212573. DOI: 10.1093/genetics/67.4.579. View

12.

Richmond R, Gilbert D, Sheehan K, Gromko M, Butterworth F . Esterase 6 and reproduction in Drosophila melanogaster. Science. 1980; 207(4438):1483-5. DOI: 10.1126/science.6767273. View

13.

Coyne J, Felton A, Lewontin R . Extent of genetic variation at a highly polymorphic esterase locus in Drosophila pseudoobscura. Proc Natl Acad Sci U S A. 1978; 75(10):5090-3. PMC: 336269. DOI: 10.1073/pnas.75.10.5090. View

14.

Cobbs G . Polymorphism for dimerizing ability at the esterase-5 locus in Drosophila pseudoobscura. Genetics. 1976; 82(1):53-62. PMC: 1213445. DOI: 10.1093/genetics/82.1.53. View

15.

Kojima K, Gillespie J, Toari Y . A profile of Drosophila species' enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes. Biochem Genet. 1970; 4(5):627-37. DOI: 10.1007/BF00486100. View

16.

Prakash H, Reddy G . Variability of alpha & beta esterase activities in the ontogeny of Drosophila rajasekari. Indian J Exp Biol. 1978; 16(5):546-8. View

17.

PUCK T, CIECIURA S, Robinson A . Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958; 108(6):945-56. PMC: 2136918. DOI: 10.1084/jem.108.6.945. View

18.

Kambysellis M, Johnson F, Richardson R . Isozyme variability in species of the genus Drosophila. IV. Distribution of the esterases in the body tissues of D. aldrichi and D. mulleri adults. Biochem Genet. 1968; 1(3):249-65. DOI: 10.1007/BF00485180. View

19.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

20.

Ahmad S . Localization of aliesterase and acetylcholinesterase enzymes in various tissues of susceptible and organophosphate resistant Musca domestica L. Comp Gen Pharmacol. 1970; 1(3):273-9. DOI: 10.1016/0010-4035(70)90020-0. View