Determination of Alpha-tocopherol in Tissues and Plasma by High-performance Liquid Chromatography

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1983 Apr 1

PMID 6869795

Citations 21

Authors

B J Zaspel

A S Csallany

Affiliations

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Abstract

A rapid and sensitive method was developed for the quantitative determination of alpha-tocopherol in tissues and plasma of rats and mice. Tissue and plasma were extracted in acetone and chromatographed on a reverse-phase C18 column with 2% water in methanol. Fluorescence and ultraviolet detection were used for tissue and plasma alpha-tocopherol levels, respectively. Extraction of tissues and plasma was found to be more complete in acetone than in other solvent systems analyzed. The average recovery of alpha-tocopherol added to tissue samples was 97%. As little as 0.1 g of tissue or 0.1 ml plasma can be accurately used for analysis. The method is sensitive to 0.05 micrograms alpha-tocopherol/g tissue.

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