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Studies of Insulin Resistance in Adipocytes Induced by Macrophage Mediator

Overview
Journal J Exp Med
Specialties Allergy & Immunology
General Medicine
Date 1983 Apr 1
PMID 6833952
Citations 36
Authors
P Pekala
M Kawakami
W Vine
M D Lane
A Cerami
Affiliations
Soon will be listed here.
Abstract

An apparent insulin resistance is noted in 3T3-L1 adipocytes after the addition of an endotoxin-induced mediator from macrophages. Examination at the level of the insulin receptor has revealed that the mediator does not effect either the functional ability of the cells to bind insulin or the ability of insulin to stimulate the uptake of glucose. The resistance appears to reflect a post-receptor interference with the insulin-induced biosynthesis of the anabolic enzymes, acetyl Co-A carboxylase and fatty acid synthetase, which are required for the conversion of glucose into storage lipid. These studies offer a new in vitro model for investigating the molecular basis of insulin resistance.

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References
1.
Gavin 3rd J, Roth J, Neville Jr D, De Meyts P, Buell D . Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc Natl Acad Sci U S A. 1974; 71(1):84-8. PMC: 387937. DOI: 10.1073/pnas.71.1.84. View
2.
Beisel W . Metabolic response to infection. Annu Rev Med. 1975; 26:9-20. DOI: 10.1146/annurev.me.26.020175.000301. View
3.
Mackall J, Student A, Polakis S, Lane M . Induction of lipogenesis during differentiation in a "preadipocyte" cell line. J Biol Chem. 1976; 251(20):6462-4. View
4.
Reed B, Kaufmann S, Mackall J, Student A, Lane M . Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes. Proc Natl Acad Sci U S A. 1977; 74(11):4876-80. PMC: 432059. DOI: 10.1073/pnas.74.11.4876. View
5.
Richardson D, Czech M . Primary role of decreased fatty acid synthesis in insulin resistance of large rat adipocytes. Am J Physiol. 1978; 234(2):E182-9. DOI: 10.1152/ajpendo.1978.234.2.E182. View