New Synthesis of a Platelet-specific Protein: Platelet Factor 4 Synthesis in a Megakaryocyte-enriched Rabbit Bone Marrow Culture System

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1983 Feb 1

PMID 6833369

Citations 13

Authors

R Ryo

A Nakeff

S S Huang

M Ginsberg

T F Deuel

Affiliations

Soon will be listed here.

Abstract

The site of synthesis of platelet-specific proteins remains to be established. With the use of short-term megakaryocyte-enriched cultures, direct evidence was obtained to show that megakaryocytes synthesize the platelet-specific protein, platelet factor 4. A megakaryocyte-enriched fraction of rabbit bone marrow for culture was obtained by centrifugal elutriation and cultured with [3H]leucine. Newly synthesized 3H-platelet factor 4 was sought by copurification with added carrier rabbit platelet factor 4, using heparin agarose affinity chromatography and immunoprecipitation with specific goat anti-rabbit platelet factor 4 antisera. SDS PAGE of the washed immunoprecipitates demonstrated a [3H]leucine-containing peak which migrated identically with purified homogeneous rabbit platelet factor 4. A second, slightly larger molecular-weight protein was identified in the gels also, suggesting that rabbit platelet factor 4 may be synthesized as a larger molecular-weight precursor in rabbit megakaryocytes. These results provide direct evidence that the platelet-specific protein, platelet factor 4, is synthesized in rabbit megakaryocytes before it is packaged into alpha-granules for release in circulating platelets.

Citing Articles

Role of platelet chemokines, PF-4 and CTAP-III, in cancer biology.

Pilatova K, Greplova K, Demlova R, Bencsikova B, Klement G, Zdrazilova-Dubska L J Hematol Oncol. 2013; 6:42.

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Optimal techniques for the immunocytochemical demonstration of beta-thromboglobulin, platelet factor 4, and fibrinogen in the alpha granules of unstimulated platelets.

Stenberg P, Shuman M, Levine S, BAINTON D Histochem J. 1984; 16(9):983-1001.

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Expression of platelet membrane glycoproteins and alpha-granule proteins by a human erythroleukemia cell line (HEL).

Tabilio A, Rosa J, Testa U, Kieffer N, Nurden A, Del Canizo M EMBO J. 1984; 3(2):453-9.

PMID: 6201359 PMC: 557365. DOI: 10.1002/j.1460-2075.1984.tb01827.x.

Biosynthesis of factor V in isolated guinea pig megakaryocytes.

Chiu H, Schick P, Colman R J Clin Invest. 1985; 75(2):339-46.

PMID: 3973008 PMC: 423488. DOI: 10.1172/JCI111706.

Structure of the rat platelet factor 4 gene: a marker for megakaryocyte differentiation.

Doi T, Greenberg S, Rosenberg R Mol Cell Biol. 1987; 7(2):898-904.

PMID: 3821732 PMC: 365148. DOI: 10.1128/mcb.7.2.898-904.1987.

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