Distribution of Fibrinogen, and Platelet Factors 4 and XIII in Subcellular Fractions of Human Platelets

Overview

Journal Br J Haematol

Specialty Hematology

Date 1975 Sep 1

PMID 1212435

Citations 13

Authors

M J Broekman

R I Handin

P Cohen

Affiliations

Soon will be listed here.

Abstract

The distribution of fibrinogen, platelet factor 4 (heparin-neutralizing factor) and factor XIII amongst subcellular fractions of human platelets was determined. It was found that fibrinogen and platelet factor 4 peaked sharply in the region of the density gradient previously shown to be heavily enriched with alpha-granules. By contrast, factor XIII, fibrin-stabilizing factor, was found exclusively in the supernatant.

Citing Articles

Defective alpha-granule production in megakaryocytes from gray platelet syndrome: ultrastructural studies of bone marrow cells and megakaryocytes growing in culture from blood precursors.

BRETON-GORIUS J, Vainchenker W, Nurden A, Levy-Toledano S, Caen J Am J Pathol. 1981; 102(1):10-9.

PMID: 7468753 PMC: 1903440.

Subcellular localization and secretion of factor V from human platelets.

Chesney C, Pifer D, Colman R Proc Natl Acad Sci U S A. 1981; 78(8):5180-4.

PMID: 6946465 PMC: 320365. DOI: 10.1073/pnas.78.8.5180.

New synthesis of a platelet-specific protein: platelet factor 4 synthesis in a megakaryocyte-enriched rabbit bone marrow culture system.

Ryo R, Nakeff A, Huang S, Ginsberg M, Deuel T J Cell Biol. 1983; 96(2):515-20.

PMID: 6833369 PMC: 2112287. DOI: 10.1083/jcb.96.2.515.

High-molecular weight kininogen. A secreted platelet protein.

Schmaier A, Zuckerberg A, SILVERMAN C, Kuchibhotla J, Tuszynski G, Colman R J Clin Invest. 1983; 71(5):1477-89.

PMID: 6406551 PMC: 437012. DOI: 10.1172/jci110901.

Optimal techniques for the immunocytochemical demonstration of beta-thromboglobulin, platelet factor 4, and fibrinogen in the alpha granules of unstimulated platelets.

Stenberg P, Shuman M, Levine S, BAINTON D Histochem J. 1984; 16(9):983-1001.

PMID: 6207144 DOI: 10.1007/BF01003853.