Establishment of Functional Human Pituitary Tumor Cell Cultures

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1982 Jan 1

PMID 6820653

Citations 4

Authors

S Melmed

D Odenheimer

H E Carlson

J M Hershman

Affiliations

Soon will be listed here.

Abstract

Five primary human pituitary tumor cell cultures were initiated from adenoma fragments obtained from patients with prolactin-secreting adenomas and acromegaly. Functional cell cultures were maintained and propagated in monolayer or suspension culture for up to 9 months. Optimal cell viability and growth were achieved using Ham's F10 medium enriched with 20% fetal bovine serum, although cells from a patient with acromegaly also grew in serum-free, defined, hormone-containing medium. Bromocriptine (100 ng/ml) did not alter the growth curve of replicating cells derived from a patient with acromegaly. These cells initially secreted 5.5 micrograms human growth hormone/10(6) cells, and hormone production diminished after 6 wk. Prolactin secretion by cells derived from prolactinomas (0.5 to 1.3 micrograms/10(6) cells/24 h) was stimulated by thyrotropin-releasing hormone (10 ng/ml) in two of the cultures. Both dopamine (10 ng/ml) and nickel chloride (1 mM) suppressed PRL secretion. These studies demonstrate that responsive human pituitary tumor cell cultures can be initiated and maintained.

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Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells.

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A Serum-Free System for Primary Cultures of Human Pituitary Adenomas.

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