Opal Suppressor Serine TRNAs from Bovine Liver Form Phosphoseryl-tRNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Oct 1

PMID 6815648

Citations 51

Authors

D HATFIELD

A Diamond

B Dudock

Affiliations

Soon will be listed here.

Abstract

An unusual minor species of bovine liver serine tRNA has previously been isolated, sequenced, and found to suppress the UGA termination codon in protein synthesis in vitro [Diamond, A., Dudock, B. & Hatfield, D. (1981) Cell 25, 497-506]. We have now found that this tRNA can be a substrate in a specific phosphorylation reaction in which phosphoseryl-tRNA is formed. Moreover, bovine liver contains a second UGA suppressor serine tRNA (tRNASerNCA; N is a modified nucleoside) which also forms phosphoseryl-tRNA. The nucleotide sequence and coding properties of tRNASerNCA are presented.

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