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A Complete Separation of Dimethylaminoazobenzenesulphonyl-amino Acids. Amino Acid Analysis with Low Nanogram Amounts of Polypeptide with Dimethylaminoazobenzenesulphonyl Chloride

Overview
Journal Biochem J
Specialty Biochemistry
Date 1982 Jun 1
PMID 6810876
Citations 8
Authors
J Y Chang
R Knecht
D G Braun
Affiliations
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Abstract

A chromatographical system has been developed to give a complete baseline separation of all dimethylaminoazobenzenesulphonyl (DABS)-amino acids for high-sensitivity amino acid analysis [Chang, Knecht & Braun (1981) Biochem. J. 199, 547--556]. The system, which uses a Merck RP-18 column with phosphate buffer (12 mM, pH 6.5)/acetonitrile mixture, allows reliable analysis of DABS-amino acids at the 1--2 pmol level. The accuracy of this new system is demonstrated by the composition analysis of two immunoglobulin light chains (214 amino acid residues) with differences at only three amino acid residue positions.

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References
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Chang J, Knecht R, Braun D . Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides. Biochem J. 1981; 199(3):547-55. PMC: 1163409. DOI: 10.1042/bj1990547. View
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