A New Isotype Sequence (V Kappa 27) of the Variable Region of Kappa-light Chains from a Mouse Hybridoma-derived Anti-(streptococcal Group A Polysaccharide) Antibody Containing an Additional Cysteine Residue. Application of The...

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Apr 1

PMID 6409088

Citations 5

Authors

J Y Chang

H Herbst

R Aebersold

D G Braun

Affiliations

Soon will be listed here.

Abstract

The first complete sequence of the variable region of a kappa-light chain (V kappa) from a mouse anti-(streptococcal group A polysaccharide) antibody (immunoglobulin 7S34.1) is reported. Immunoglobulin 7S34.1 was isolated from the ascitic fluid of hybridoma 7S34.1 previously cloned in vitro. A newly developed technique for the isolation of peptides by using pre-column formation of peptide derivatives with dimethylaminoazobenzene isothiocyanate also served to complete the sequence. The sequence of the variable region of the kappa-light chain of immunoglobulin 7S34.1 defines a new mouse V kappa isotype (V kappa 27) and is the first mouse immunoglobulin light-chain variable region to be shown to have an extra cysteine residue at position 48.

Citing Articles

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