I-region Genetic Restrictions Imposed Upon the Recognition of KLH by Murine T-cell Clones

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1981 Jan 1

PMID 6800947

Citations 7

Authors

M Shigeta

C G Fathman

Affiliations

Soon will be listed here.

Abstract

Data presented in this paper show that the recognition of keyhole limpet hemocyanin by murine T-cell clones is restricted by products of the I region. These data have been obtained by genetic mapping studies as well as by the use of monoclonal Ia-specific antibodies which inhibit the ability of antigen-presenting cells to effectively present antigen to such T- cell clones. Use of heterozygous antigen-presenting cells derived from crosses between B6.C-H-2bm12 and B10.A(4R) mice have allowed us to show that both trans-complementing I-A products are used for restriction of recognition of KLH. These data were confirmed using monoclonal Ia antibodies to inhibition recognition of KLH by the same T -cell clones. Thus, we have shown that there exist hybrid molecules formed by free combinatorial association of products encoded within the I-A subregion which restrict the recognition of soluble antigen. Additionally, we have shown that the molecule formed by complementation between the alpha chain encoded within the I-E region and a beta chain encoded within the A region (Ae) can fuction effectively in presenting KLH to certain murine T -cell clones. These results support the hypothesis that the recognition of individual antigenic epitopes within large multideterminant antigens is under the control of Ir genes.

Citing Articles

Role of the major histocompatibility complex in T cell activation of B cell subpopulations. A single monoclonal T helper cell population activates different B cell subpopulations by distinct pathways.

Asano Y, Shigeta M, Fathman C, Singer A, Hodes R J Exp Med. 1982; 156(2):350-60.

PMID: 6980253 PMC: 2186748. DOI: 10.1084/jem.156.2.350.

Involvement of Mhc loci in immune responses that are not Ir-gene-controlled.

Marusic M, Nagy Z, Koszinowski U, Klein J Immunogenetics. 1982; 16(5):471-83.

PMID: 6818139 DOI: 10.1007/BF00372105.

Multiple functional sites on a single Ia molecule defined using T cell clones and antibodies with chain-determined specificity.

Frelinger J, Shigeta M, Infante A, Nelson P, Pierres M, Fathman C J Exp Med. 1984; 159(3):704-15.

PMID: 6421980 PMC: 2187252. DOI: 10.1084/jem.159.3.704.

T cell clones specific for hybrid I-A molecules. Discrimination with monoclonal anti-I-Ak antibodies.

Beck B, Frelinger J, Shigeta M, Infante A, Cummings D, Hammerling G J Exp Med. 1982; 156(4):1186-94.

PMID: 6185607 PMC: 2186826. DOI: 10.1084/jem.156.4.1186.

Beck B, Infante A, Fathman C Immunogenetics. 1982; 15(6):565-71.

PMID: 6179865 DOI: 10.1007/BF00347050.

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