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Commitment of Bacterial Spores to Germinate. A Measure of the Trigger Reaction

Overview
Journal Biochem J
Specialty Biochemistry
Date 1981 Jul 15
PMID 6798972
Citations 91
Authors
G S Stewart
K Johnstone
E Hagelberg
D J Ellar
Affiliations
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Abstract

The rate of commitment of bacterial spores to germinate after short exposure to L-alanine increases exponentially from the time of addition of L-alanine. This absence of a lag facilitates kinetic analysis and allows the dependence of commitment on temperature and pH to be determined. The pH profile of commitment has been compared with that obtained from measurements of absorbance decreases during germination, and the two profiles exhibit differing pK values. It is suggested that because the decrease in A600 of spore suspensions is a late event in germination, it is an unsuitable parameter for studying germination-triggering reactions. Commitment has been shown to be temperature-dependent, with an optimum at approx. 37 degrees C and an activation energy (mu) of 1.08 X 10(5) J/mol. The data obtained from the present studies have been used to develop a model for the triggering of germination.

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References
1.
Scott I, Ellar D . Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate. J Bacteriol. 1978; 135(1):133-7. PMC: 224788. DOI: 10.1128/jb.135.1.133-137.1978. View
2.
Woese C, Vary J, HALVORSON H . A kinetic model for bacterial spore germination. Proc Natl Acad Sci U S A. 1968; 59(3):869-75. PMC: 224768. DOI: 10.1073/pnas.59.3.869. View
3.
LEVINSON H, HYATT M . Activation energy for glucose-induced germination of Bacillus megaterium spores. J Bacteriol. 1970; 103(1):269-70. PMC: 248069. DOI: 10.1128/jb.103.1.269-270.1970. View
4.
Murrell W, WILLS P . Initiation of Bacillus spore germination by hydrostatic pressure: effect of temperature. J Bacteriol. 1977; 129(3):1272-80. PMC: 235099. DOI: 10.1128/jb.129.3.1272-1280.1977. View
5.
Dring G, Gould G . Sequence of events during rapid germination of spores of Bacillus cereus. J Gen Microbiol. 1971; 65(1):101-4. DOI: 10.1099/00221287-65-1-101. View