Draft Genome of Neotropical Bacillus Thuringiensis UFT038 and Its Potential Against Lepidopteran Soybean Pests

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2023 Nov 28

PMID 38017300

Authors

Giselly B Alves

Timoteo S O Lemes

Eliseu J G Pereira

Juan L Jurat-Fuentes

Guy Smagghe

Gil R Santos

Khalid Haddi

Roberto F T Correa

Fernando L Melo

Luis O Viteri Jumbo

Eugenio E Oliveira

Antonio J Peron

Bergmann M Ribeiro

Raimundo W S Aguiar

Affiliations

Soon will be listed here.

Abstract

Bacillus thuringiensis (Bt) is known for its Cry and Vip3A pesticidal proteins with high selectivity to target pests. Here, we assessed the potential of a novel neotropical Bt strain (UFT038) against six lepidopteran pests, including two Cry-resistant populations of fall armyworm, Spodoptera frugiperda. We also sequenced and analyzed the genome of Bt UFT038 to identify genes involved in insecticidal activities or encoding other virulence factors. In toxicological bioassays, Bt UFT038 killed and inhibited the neonate growth in a concentration-dependent manner. Bt UFT038 and HD-1 were equally toxic against S. cosmioides, S. frugiperda (S_Bt and R_Cry1 + 2Ab populations), Helicoverpa zea, and H. armigera. However, larval growth inhibition results indicated that Bt UFT038 was more toxic than HD-1 to S. cosmioides, while HD-1 was more active against Chrysodeixis includens. The draft genome of Bt UFT038 showed the cry1Aa8, cry1Ac11, cry1Ia44, cry2Aa9, cry2Ab35, and vip3Af5 genes. Besides this, genes encoding the virulence factors (inhA, plcA, piplC, sph, and chi1-2) and toxins (alo, cytK, hlyIII, hblA-D, and nheA-C) were also identified. Collectively, our findings reveal the potential of the Bt UFT038 strain as a source of insecticidal genes against lepidopteran pests, including S. cosmioides and S. frugiperda.

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