Ethylenediaminetetraacetate-extractable Protein-lipopolysaccharide Complex of Pseudomonas Aeruginosa: Characterization of Protein Components

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Dec 1

PMID 6796566

Citations 3

Authors

R C Hedstrom

R K Schockley

R G Eagon

Affiliations

Soon will be listed here.

Abstract

Five major outer membrane proteins (D1, D2, E, G, and H1) of Pseudomonas aeruginosa, but not proteins F (porin), I (lipoprotein), and H2, were detected in high-molecular-weight protein-lipopolysaccharide complex(es) solubilized from sucrose-stabilized cells on exposure to ethylenediaminetetraacetate and tris(hydroxymethyl)aminomethane.

Citing Articles

PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A.

Moskowitz S, Ernst R, Miller S J Bacteriol. 2004; 186(2):575-9.

PMID: 14702327 PMC: 305751. DOI: 10.1128/JB.186.2.575-579.2004.

Antibody response of infected mice to outer membrane proteins of Pseudomonas aeruginosa.

Hedstrom R, PAVLOVSKIS O, Galloway D Infect Immun. 1984; 43(1):49-53.

PMID: 6418663 PMC: 263386. DOI: 10.1128/iai.43.1.49-53.1984.

Immunochemical and biochemical analysis of the polyvalent Pseudomonas aeruginosa vaccine PEV.

Macintyre S, McVeigh T, Owen P Infect Immun. 1986; 51(2):675-86.

PMID: 3080373 PMC: 262404. DOI: 10.1128/iai.51.2.675-686.1986.

References

Markwell M, Haas S, Bieber L, Tolbert N . A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1):206-10. DOI: 10.1016/0003-2697(78)90586-9. View

Gmeiner J, Schlecht S . Molecular composition of the outer membrane of Escherichia coli and the importance of protein-lipopolysaccharide interactions. Arch Microbiol. 1980; 127(2):81-6. DOI: 10.1007/BF00428010. View

Datta D, Arden B, Henning U . Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors. J Bacteriol. 1977; 131(3):821-9. PMC: 235537. DOI: 10.1128/jb.131.3.821-829.1977. View

Yu F, Mizushima S . Stimulation by lipopolysaccharide of the binding of outer membrane proteins O-8 and O-9 to the peptidoglycan layer of Escherichia coli K--12. Biochem Biophys Res Commun. 1977; 74(4):1397-402. DOI: 10.1016/0006-291x(77)90597-6. View

Gilleland Jr H, Stinnett J, Eagon R . Ultrastructural and chemical alteration of the cell envelope of Pseudomonas aeruginosa, associated with resistance to ethylenediaminetetraacetate resulting from growth in a Mg2+-deficient medium. J Bacteriol. 1974; 117(1):302-11. PMC: 246557. DOI: 10.1128/jb.117.1.302-311.1974. View

Hancock R, Nikaido H . Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J Bacteriol. 1978; 136(1):381-90. PMC: 218670. DOI: 10.1128/jb.136.1.381-390.1978. View

ROGERS S, Gilleland Jr H, Eagon R . Characterization of a protein-lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid. Can J Microbiol. 1969; 15(7):743-8. DOI: 10.1139/m69-130. View

Schweizer M, Hindennach I, Garten W, Henning U . Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur J Biochem. 1978; 82(1):211-7. DOI: 10.1111/j.1432-1033.1978.tb12013.x. View

Nikaido H, Nakae T . The outer membrane of Gram-negative bacteria. Adv Microb Physiol. 1979; 20:163-250. DOI: 10.1016/s0065-2911(08)60208-8. View

10.

Repaske R . Lysis of gram-negative organisms and the role of versene. Biochim Biophys Acta. 1958; 30(2):225-32. DOI: 10.1016/0006-3002(58)90044-1. View

11.

Hancock R, Irvin R, Costerton J, Carey A . Pseudomonas aeruginosa outer membrane: peptidoglycan-associated proteins. J Bacteriol. 1981; 145(1):628-31. PMC: 217314. DOI: 10.1128/jb.145.1.628-631.1981. View

12.

Stinnett J, Eagon R . Outer (cell wall) membrane proteins of Pseudomonas aeruginosa. Can J Microbiol. 1973; 19(12):1469-71. DOI: 10.1139/m73-239. View

13.

Gilleland Jr H, Stinnett J, Roth I, Eagon R . Freeze-etch study of Pseudomonas aeruginosa: localization within the cell wall of an ethylenediaminetetraacetate-extractable. J Bacteriol. 1973; 113(1):417-32. PMC: 251645. DOI: 10.1128/jb.113.1.417-432.1973. View

14.

Stinnett J, Eagon R . A model system for studying protein-lipopolysaccharide synthesis, assembly, and insertion in the outer membrane of Pseudomonas aeruginosa. Can J Microbiol. 1975; 21(11):1834-41. DOI: 10.1139/m75-266. View

15.

Day D, Ingram J . Protein-lipopolysaccharide interactions. 1. The reaction of lysozyme with Pseudomonas aeruginosa LPS. Can J Microbiol. 1978; 24(2):196-9. DOI: 10.1139/m78-035. View

16.

Eagon R, Carson K . LYSIS OF CELL WALLS AND INTACT CELLS OF PSEUDOMONAS AERUGINOSA BY ETHYLENEDIAMINE TETRAACETIC ACID AND BY LYSOZYME. Can J Microbiol. 1965; 11:193-201. DOI: 10.1139/m65-025. View

17.

Eagon R, Phibbs Jr P . Kinetics of transport of glucose, fructose, and mannitol by Pseudomonas aeruginosa. Can J Biochem. 1971; 49(9):1031-41. DOI: 10.1139/o71-151. View

18.

Stinnett J, Gilleland Jr H, Eagon R . Proteins released from cell envelopes of Pseudomonas aeruginosa on exposure to ethylenediaminetetraacetate: comparison with dimethylformamide-extractable proteins. J Bacteriol. 1973; 114(1):399-407. PMC: 251778. DOI: 10.1128/jb.114.1.399-407.1973. View

19.

Yamada H, Mizushima S . Interaction between major outer membrane protein (O-8) and lipopolysaccharide in Escherichia coli K12. Eur J Biochem. 1980; 103(1):209-18. DOI: 10.1111/j.1432-1033.1980.tb04305.x. View

20.

Hancock R, Carey A . Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins. J Bacteriol. 1979; 140(3):902-10. PMC: 216732. DOI: 10.1128/jb.140.3.902-910.1979. View