Terminal Branching of the Respiratory Electron Transport Chain in Neisseria Meningitidis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Jun 1

PMID 6769915

Citations 6

Authors

E K Yu

I W DeVoe

Affiliations

Soon will be listed here.

Abstract

The respiratory components of the envelope membrane preparation of Neisseria meningitidis were investigated. Oxidase activities were demonstrated in this fraction in the presence of succinic acid, reduced nicotinamide adenine dinucleotide, and ascorbate-N,N,N',N'-tetramethyl-p-phenylene-diamine (TMPD). Differences in the kinetics of inhibition by terminal oxidase inhibitors on the three oxidase activities indicated that ascorbate-TMPD oxidation involved only an azide-sensitive oxidase, whereas oxidation of the physiological substrates involved two oxidases, one of which was relatively azide resistant. Spectrophotometric studies revealed that ascorbate-TMPD donated its electrons exclusively to cytochrome o, whereas the physiological substrates were oxidized via both cytochromes o and a. The effects of class II inhibitors on the oxidases suggest terminal branching of the electron transport chain at the cytochrome b level. A model of the respiratory system in N. meningitidis is proposed.

Citing Articles

Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens.

Jaramillo-Lanchero R, Suarez-Alvarez P, Teheran-Sierra L Sci Rep. 2021; 11(1):8987.

PMID: 33903681 PMC: 8076288. DOI: 10.1038/s41598-021-88388-0.

Organization of the electron transfer chain to oxygen in the obligate human pathogen Neisseria gonorrhoeae: roles for cytochromes c4 and c5, but not cytochrome c2, in oxygen reduction.

Li Y, Hopper A, Overton T, Squire D, Cole J, Tovell N J Bacteriol. 2010; 192(9):2395-406.

PMID: 20154126 PMC: 2863483. DOI: 10.1128/JB.00002-10.

Modeling Neisseria meningitidis metabolism: from genome to metabolic fluxes.

E Baart G, Zomer B, de Haan A, van der Pol L, Beuvery E, Tramper J Genome Biol. 2007; 8(7):R136.

PMID: 17617894 PMC: 2323225. DOI: 10.1186/gb-2007-8-7-r136.

L-cysteine oxidase activity in the membrane of Neisseria meningitidis.

Yu E, DeVoe I J Bacteriol. 1981; 145(1):280-7.

PMID: 6780513 PMC: 217270. DOI: 10.1128/jb.145.1.280-287.1981.

The meningococcus and mechanisms of pathogenicity.

DeVoe I Microbiol Rev. 1982; 46(2):162-90.

PMID: 6126800 PMC: 281537. DOI: 10.1128/mr.46.2.162-190.1982.

References

Jones M . Physiological role for the membrane bound ascorbate-TMPD oxidase in pseudomonas putida. Arch Microbiol. 1975; 102(3):275-9. DOI: 10.1007/BF00428378. View

Niven D, Collins P, Knowles C . The respiratory system of Chromobacterium violaceum grown under conditions of high and low cyanide evolution. J Gen Microbiol. 1975; 90(2):271-85. DOI: 10.1099/00221287-90-2-271. View

DeVoe I . Egestion of degraded meningococci by polymorphonuclear leukocytes. J Bacteriol. 1976; 125(1):258-66. PMC: 233359. DOI: 10.1128/jb.125.1.258-266.1976. View

DeVoe I, Golchrist J . Localization of tetramethylphenylenediamine-oxidase in the outer cell wall layer of Neisseria meningitidis. J Bacteriol. 1976; 128(1):144-8. PMC: 232836. DOI: 10.1128/jb.128.1.144-148.1976. View

Kenimer E, Lapp D . Effects of selected inhibitors on electron transport in Neisseria gonorrhoeae. J Bacteriol. 1978; 134(2):537-45. PMC: 222284. DOI: 10.1128/jb.134.2.537-545.1978. View

DeVoe I, Gilchrist J . Piliation and colonial morphology among laboratory strains of meningococci. J Clin Microbiol. 1978; 7(4):379-84. PMC: 274971. DOI: 10.1128/jcm.7.4.379-384.1978. View

Archibald F, DeVoe I . Iron in Neisseria meningitidis: minimum requirements, effects of limitation, and characteristics of uptake. J Bacteriol. 1978; 136(1):35-48. PMC: 218629. DOI: 10.1128/jb.136.1.35-48.1978. View

Morse S . The biology of the gonococcus. CRC Crit Rev Microbiol. 1978; 7(2):93-189. DOI: 10.3109/10408417909083071. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

10.

BEERS Jr R, SIZER I . A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952; 195(1):133-40. View

11.

Dowd J, RIGGS D . A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965; 240:863-9. View

12.

Winter D, Morse S . Physiology and metabolism of pathogenic Neisseria: partial characterization of the respiratory chain of Neisseria gonorrhoeae. J Bacteriol. 1975; 123(2):631-6. PMC: 235769. DOI: 10.1128/jb.123.2.631-636.1975. View

13.

Jones C, REDFEARN E . Electron transport in Azotobacter vinelandii. Biochim Biophys Acta. 1966; 113(3):467-81. DOI: 10.1016/s0926-6593(66)80005-x. View

14.

Jurtshuk P, Aston P, Old L . Enzymatic oxidation of tetramethyl-p-phenylenediamine and p-phenylenediamine by the electron transport particulate fraction of Azotobacter vinelandii. J Bacteriol. 1967; 93(3):1069-78. PMC: 276555. DOI: 10.1128/jb.93.3.1069-1078.1967. View

15.

BROBERG P, Smith L . The cytochrome system of Bacillus megaterium KM. The presence and some properties of two CO-binding cytochromes. Biochim Biophys Acta. 1967; 131(3):479-89. DOI: 10.1016/0005-2728(67)90007-2. View

16.

Appleby C . Electron transport systems of Rhizobium japonicum. I. Haemoprotein P-450, other CO-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochim Biophys Acta. 1969; 172(1):71-87. DOI: 10.1016/0005-2728(69)90093-0. View

17.

Sasaki T, MOTOKAWA Y, KIKUCHI G . Occurrence of both a-type and o-type cytochromes as the functional terminal oxidases in Rhodopseudomonas spheroides. Biochim Biophys Acta. 1970; 197(2):284-91. DOI: 10.1016/0005-2728(70)90039-3. View

18.

Cheng K, Ingram J, Costerton J . Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. J Bacteriol. 1970; 104(2):748-53. PMC: 285053. DOI: 10.1128/jb.104.2.748-753.1970. View

19.

Jones M, Hughes D . The oxidation of nicotinic acid by Pseudomonas ovalis Chester. The terminal oxidase. Biochem J. 1972; 129(3):755-61. PMC: 1174177. DOI: 10.1042/bj1290755. View

20.

White D, Sinclair P . Branched electron-transport systems in bacteria. Adv Microb Physiol. 1971; 5:173-211. DOI: 10.1016/s0065-2911(08)60407-5. View