Enzymatic Oxidation of Tetramethyl-p-phenylenediamine and P-phenylenediamine by the Electron Transport Particulate Fraction of Azotobacter Vinelandii

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1967 Mar 1

PMID 6025414

Citations 14

Authors

P Jurtshuk

P R Aston

L Old

Affiliations

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Abstract

The ability of the electron transport particulate fraction of Azotobacter vinelandii strain O to oxidize tetramethyl-p-phenylenediamine (TMPD) and p-phenylenediamine (PPD) was examined in detail. The highest specific activity for TMPD and PPD oxidation concentrated in the A. vinelandii O R(3) fraction. The A. vinelandii O R(3) fraction was used to develop a standard manometric assay which gave optimal oxidation rates for both of these dyes. The conditions of the assay and all essential related enzymatic kinetic parameters are presented. Other para derivatives of phenylenediamines also were oxidized readily, whereas ortho and meta derivatives were not. Hydroquinone, p-hydroxybenzoic acid, p-cresol, tyrosine, pyrogallol, pyrocatechol, and diphenylamine were not able to serve as electron donors for the A. vinelandii O R(3) system. The probable involvement of a particle-bound cytochrome oxidase is indicated by the marked sensitivity of both TMPD and PPD oxidation to cyanide, axide, phenylhydrazine, hydroxylamine, and, to a lesser degree, carbon monoxide.

Citing Articles

Comparative Cytochrome Oxidase and Superoxide Dismutase Analyses on Strains of Azotobacter vinelandii and Other Related Free-Living Nitrogen-Fixing Bacteria.

Jurtshuk P, Liu J, Moore E Appl Environ Microbiol. 1984; 47(5):1185-7.

PMID: 16346548 PMC: 240103. DOI: 10.1128/aem.47.5.1185-1187.1984.

Terminal branching of the respiratory electron transport chain in Neisseria meningitidis.

Yu E, DeVoe I J Bacteriol. 1980; 142(3):879-87.

PMID: 6769915 PMC: 294113. DOI: 10.1128/jb.142.3.879-887.1980.

Activation studies by phospholipids on the purified cytochrome c4:o oxidase of Azotobacter vinelandii.

Wong T, JURTSHUK Jr P J Bioenerg Biomembr. 1984; 16(5-6):477-89.

PMID: 6100376 DOI: 10.1007/BF00743240.

Microtubule in Azotobacter vinelandii strain O.

Pope L, Jurtshuk P J Bacteriol. 1967; 94(6):2062-4.

PMID: 6074408 PMC: 276940. DOI: 10.1128/jb.94.6.2062-2064.1967.

Phospholipids of Azotobacter vinelandii.

Jurtshuk P, Schlech B J Bacteriol. 1969; 97(3):1507-8.

PMID: 5776538 PMC: 249880. DOI: 10.1128/jb.97.3.1507-1508.1969.

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