Identification of the Origins of T4 DNA Replication

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Dec 1

PMID 6760193

Citations 10

Authors

G J King

W M Huang

Affiliations

Soon will be listed here.

Abstract

Two physical origins of T4 DNA replication were determined by hybridization of viral DNA prepared 2.5 min after infection to a display of total T4 DNA. This is the earliest time after T4 infection of Escherichia coli at 37 degrees C that labeled and hybridizable DNA can be detected. The two origins, separated by about 25 kilobases, were identified and localized in the early region of the T4 map. One of them is located in a 5.6-kilobase EcoRI fragment containing genes 62-46. The other is located between genes rI and e in a 1.9-kilobase EcoRI fragment. Both of these T4 fragments have been cloned and their interactions with the host cell are discussed.

Citing Articles

Multiple origins of replication contribute to a discontinuous pattern of DNA synthesis across the T4 genome during infection.

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Two new early bacteriophage T4 genes, repEA and repEB, that are important for DNA replication initiated from origin E.

Vaiskunaite R, Miller A, Davenport L, Mosig G J Bacteriol. 1999; 181(22):7115-25.

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Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.

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Genetic analysis of dar, uvsW, and uvsY in bacteriophage T4: dar and uvsW are alleles.

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Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4.

Radany E, Naumovski L, Love J, Gutekunst K, Hall D, Friedberg E J Virol. 1984; 52(3):846-56.

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