Locations of Bacteriophage T4 Origins of Replication

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1985 May 1

PMID 3989906

Citations 7

Authors

J K Yee

R C Marsh

Affiliations

Soon will be listed here.

Abstract

Partially replicated bacteriophage T4 DNA containing cytosine was isolated from cells 6.5 and 7 min after infection and cleaved with restriction endonuclease BglII or BamHI. Positions of replication eyes relative to the cleavage sites were observed by electron microscopy. Four groups of eyes were found. They are consistent with replication from origins located at map positions 34, 60, 73, and 86 kilobases. In individual molecules that contained two or three eyes, the distribution of the eyes agreed with the initiation of replication at more than one of these four assigned origins and possibly at two additional origins located near 15 and 110 kilobases, which were reported by P. M. Macdonald, R. M. Seaby, W. Brown, and G. Mosig (p. 111-116, in D. Schlessinger, ed., Microbiology--1983, 1983) and M. E. Halpern, T. Mattson, and A. W. Kozinski (Proc. Natl. Acad. Sci. U.S.A. 76:6137-6141, 1979).

Citing Articles

Multiple origins of replication contribute to a discontinuous pattern of DNA synthesis across the T4 genome during infection.

Brister J, Nossal N J Mol Biol. 2007; 368(2):336-48.

PMID: 17346743 PMC: 1934900. DOI: 10.1016/j.jmb.2007.02.008.

Bacteriophage T4 genome.

Miller E, Kutter E, Mosig G, Arisaka F, Kunisawa T, Ruger W Microbiol Mol Biol Rev. 2003; 67(1):86-156, table of contents.

PMID: 12626685 PMC: 150520. DOI: 10.1128/MMBR.67.1.86-156.2003.

Two new early bacteriophage T4 genes, repEA and repEB, that are important for DNA replication initiated from origin E.

Vaiskunaite R, Miller A, Davenport L, Mosig G J Bacteriol. 1999; 181(22):7115-25.

PMID: 10559179 PMC: 94188. DOI: 10.1128/JB.181.22.7115-7125.1999.

Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4.

Valerie K, Stevens J, Lynch M, Henderson E, de Riel J Nucleic Acids Res. 1986; 14(21):8637-54.

PMID: 3024113 PMC: 311883. DOI: 10.1093/nar/14.21.8637.

Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

Stitt B, Mosig G J Bacteriol. 1989; 171(7):3872-80.

PMID: 2544560 PMC: 210138. DOI: 10.1128/jb.171.7.3872-3880.1989.

References

Wood W . Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J Mol Biol. 1966; 16(1):118-33. DOI: 10.1016/s0022-2836(66)80267-x. View

Mosig G . A preferred origin and direction of bacteriophage T4 DNA replication. I. A gradient of allele frequencies in crosses between normal and small T4 particles. J Mol Biol. 1970; 53(3):503-14. DOI: 10.1016/0022-2836(70)90080-x. View

Marsh R, Breschkin A, Mosig G . Origin and direction of bacteriophage T4 DNA replication. II. A gradient of marker frequencies in partially replicated T4 DNA as assayed by transformation. J Mol Biol. 1971; 60(2):213-33. DOI: 10.1016/0022-2836(71)90289-0. View

Delius H, Howe C, Kozinski A . Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971; 68(12):3049-53. PMC: 389588. DOI: 10.1073/pnas.68.12.3049. View

Mosig G, Ehring R, Schliewen W, Bock S . The patterns of recombination and segregation in terminal regions of T4DNA molecules. Mol Gen Genet. 1971; 113(1):51-91. DOI: 10.1007/BF00335007. View

Kim J, Davidson N . Electron microscope heteroduplex study of sequence relations of T2, T4, and T6 bacteriophage DNAs. Virology. 1974; 57(1):93-111. DOI: 10.1016/0042-6822(74)90111-1. View

Snyder L, Gold L, Kutter E . A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA. Proc Natl Acad Sci U S A. 1976; 73(9):3098-102. PMC: 430943. DOI: 10.1073/pnas.73.9.3098. View

Sutcliffe J . Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 1:77-90. DOI: 10.1101/sqb.1979.043.01.013. View

Halpern M, Mattson T, Kozinski A . Origins of phage T4 DNA replication as revealed by hybridization to cloned genes. Proc Natl Acad Sci U S A. 1979; 76(12):6137-41. PMC: 411818. DOI: 10.1073/pnas.76.12.6137. View

10.

Marsh R, Hepburn M . Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI. J Virol. 1981; 38(1):104-14. PMC: 171130. DOI: 10.1128/JVI.38.1.104-114.1981. View

11.

King G, Huang W . Identification of the origins of T4 DNA replication. Proc Natl Acad Sci U S A. 1982; 79(23):7248-52. PMC: 347316. DOI: 10.1073/pnas.79.23.7248. View

12.

Dannenberg R, Mosig G . Early intermediates in bacteriophage T4 DNA replication and recombination. J Virol. 1983; 45(2):813-31. PMC: 256476. DOI: 10.1128/JVI.45.2.813-831.1983. View