Long-chain Acyl-CoA Levels in Liver from Rats Fed High-fat Diets: is It of Significance for an Increased Peroxisomal Beta-oxidation?

The levels of long-chain acyl-CoA in the livers of rats given diets containing various amounts of dietary oils were investigated. Increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-CoA. With partially hydrogenated marine oil, a sigmoidal dose-response curve was obtained, giving a 60% increase when 20% or more of this oil was in the diet. All high-fat diets tested resulted in higher levels of long-chain acyl-CoA than the low-fat control containing soybean oil. The increase was most prominent with partially hydrogenated marine and rapeseed oils. With diets containing partially hydrogenated marine oil, the ratio of long-chain acyl-CoA to acid-soluble CoA was increased after 3 days, but decreased after 3 weeks, to a value similar to that observed in animals fed soybean oil because of an extensive increase in acid-soluble CoA. Increased levels of long-chain acyl-CoA were also observed after clofibrate was administered, but the increase was less prominent than observed with high-fat diets. When comparing the levels of long-chain acyl-CoA observed after 3 days on different diets with the peroxisomal beta-oxidation activity previously determined after 3 weeks on the corresponding diets, a straight line was obtained. These results are discussed in relation to the possibility that long-chain acyl-CoA induces peroxisomal beta-oxidation activity.