Stimulation of Glycosaminoglycan Production and Lysosomal Activity of Human Synovial Cells in Culture by Low Environmental PH

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1984 Apr 1

PMID 6712304

Citations 3

Authors

B J Clarris

J R Fraser

K D Muirden

L P Malcolm

M W Holmes

K Rogers

Affiliations

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Abstract

Glycosaminoglycan production, acid hydrolase activity, proliferation, and morphology were examined in human synovial cells subjected to low environmental pH. The amount and the molecular size of newly synthesised glycosaminoglycan (GAG) were increased without significant change in the rate of cell proliferation. Lowered pH produced an increase in the size of cytoplasmic organelles. Some of these possessed ultrastructural features of lysosomes, but others were clearly nonlysosomal and were of uncertain identity. Intracellular activity of the lysosomal acid hydrolase N-acetyl-beta-glucosaminidase (NAG) was not altered by low pH, but a marked increase occurred in extracellular NAG activity, indicating enhanced release.

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References

Fraser J, McCall J . Culture of synovial cells in vitro. Notes on isolation and propagation. Ann Rheum Dis. 1965; 24(4):351-9. PMC: 1030978. DOI: 10.1136/ard.24.4.351. View

Caygill J, Pitkeathly D . A study of beta-acetylglucosaminase and acid phosphatase in pathological joint fluids. Ann Rheum Dis. 1966; 25(2):137-44. PMC: 2453386. DOI: 10.1136/ard.25.2.137. View

Castor C, PRINCE R, Hazelton M . Hyaluronic acid in human synovial effusions; a sensitive indicator of altered connective tissue cell function during inflammation. Arthritis Rheum. 1966; 9(6):783-94. DOI: 10.1002/art.1780090606. View

Clarris B, Fraser J . The effects of homologous and heterologous whole serum upon multiplication of recently-isolated human synovial cells in culture. Aust J Exp Biol Med Sci. 1967; 45(5):549-60. DOI: 10.1038/icb.1967.54. View

Clarris B, Fraser J . Relationship between chromosomal changes and alterations in the behaviour of a strain of human synovial cells during its life history in vitro. Ann Rheum Dis. 1968; 27(6):597-603. PMC: 1010481. DOI: 10.1136/ard.27.6.597. View

Wegelius O, Klockars M, VAINIO K . Acid phosphatase activity in rheumatoid synovia. Acta Med Scand. 1968; 183(6):549-51. DOI: 10.1111/j.0954-6820.1968.tb10521.x. View

BECKMAN G, Beckman L, LEMPERG R . Acid phosphatase activity in the synovial fluid of patients with rheumatoid arthritis and other joint disorders. Acta Rheumatol Scand. 1971; 17(1):47-56. DOI: 10.3109/rhe1.1971.17.issue-1-4.07. View

Treuhaft P, McCarty D . Synovial fluid pH, lactate, oxygen and carbon dioxide partial pressure in various joint diseases. Arthritis Rheum. 1971; 14(4):475-84. DOI: 10.1002/art.1780140407. View

Muirden K . Lysosomal enzymes in synovial membrane in rheumatoid arthritis. Relationship to joint damage. Ann Rheum Dis. 1972; 31(4):265-71. PMC: 1005924. DOI: 10.1136/ard.31.4.265. View

10.

Baxter E, Fraser J, Harris G . Fractionation and recovery of secretions of synovial cells synthesized in culture with radioactive precursors. Ann Rheum Dis. 1973; 32(1):35-40. PMC: 1006031. DOI: 10.1136/ard.32.1.35. View

11.

Lie S, Schofield B, Taylor Jr H, Doty S . Structure and function of the lysosomes of human fibroblasts in culture: dependence on medium pH. Pediatr Res. 1973; 7(1):13-9. DOI: 10.1203/00006450-197301000-00003. View

12.

Kittlick P, Neupert G . [Modification of the synthesis of acid mucopolysaccharides (glycosaminoglycans) in fibroblast cultures. IV. Influence of the pH-value on cell proliferation and pattern of MPS-distribution]. Exp Pathol (Jena). 1972; 7(4):117-24. View

13.

McCord J . Free radicals and inflammation: protection of synovial fluid by superoxide dismutase. Science. 1974; 185(4150):529-31. DOI: 10.1126/science.185.4150.529. View

14.

Ceccarini C . Effect of pH on plating efficiency, serum reguirement, and incorporation of radioactive precursors into human cells. In Vitro. 1975; 11(2):78-86. DOI: 10.1007/BF02624079. View

15.

DINGLE J . The secretion of enzymes into the pericellular environment. Philos Trans R Soc Lond B Biol Sci. 1975; 271(912):315-24. DOI: 10.1098/rstb.1975.0055. View

16.

Marshall J, Fraser J, Muirden K . Lysosomal activation by neutral saccharides in cell cultures of synovium. Ann Rheum Dis. 1977; 36(2):130-8. PMC: 1006647. DOI: 10.1136/ard.36.2.130. View

17.

Eeckhout Y, Vaes G . Further studies on the activation of procollagenase, the latent precursor of bone collagenase. Effects of lysosomal cathepsin B, plasmin and kallikrein, and spontaneous activation. Biochem J. 1977; 166(1):21-31. PMC: 1164952. DOI: 10.1042/bj1660021. View

18.

Fraser J, Clarris B, Baxter E . Patterns of induced variation in the morphology, hyaluronic acid secretion, and lysosomal enzyme activity of cultured human synovial cells. Ann Rheum Dis. 1979; 38(3):287-94. PMC: 1000452. DOI: 10.1136/ard.38.3.287. View

19.

Castor C, Bignall M, Hossler P, Roberts D . Connective tissue activation. XXI. Regulation of glycosaminoglycan metabolism by lymphocyte (CTAP-I) and platelet (CTAP-III) growth factors. In Vitro. 1981; 17(9):777-85. DOI: 10.1007/BF02618444. View

20.

Clarris B, Malcolm L . Effects of prostaglandins E1, E2, and F2 alpha on N-acetyl-beta-glucosaminidase activities of human synovial cells in culture. Ann Rheum Dis. 1983; 42(2):187-91. PMC: 1001097. DOI: 10.1136/ard.42.2.187. View