The Effects of Synovial Fluid Macrophages and Interleukin-1 on Hyaluronic Acid Synthesis by Normal Synovial Fibroblasts

Overview

Journal Rheumatol Int

Specialty Rheumatology

Date 1986 Jan 1

PMID 3489274

Citations 5

Authors

K Pulkki

Affiliations

Soon will be listed here.

Abstract

The effects of peripheral blood monocyte and rheumatoid synovial fluid macrophage conditioned media were studied on hyaluronic acid (HA) metabolism of normal synovial fibroblasts. Both media stimulated HA synthesis about two-fold compared to controls (1% fetal calf serum). The activated mononuclear phagocyte conditioned media did not contain HA-degrading activity in these experiments. The effects of various concentrations of interleukin-1 (IL-1) on HA synthesis and proliferation of synovial fibroblasts were studied. Even at very low concentrations (0.1 IU IL-1/ml) HA synthesis was stimulated. With increasing concentrations HA synthesis did not increase but proliferation was stimulated. Stimulated fibroblasts synthesized mainly high molecular weight HA. Thus with IL-1-activation, normal synovial fibroblasts could not produce increased amounts of abnormal HA with decreased molecular weight.

Citing Articles

Cartilage Wear Particles Induce an Inflammatory Response Similar to Cytokines in Human Fibroblast-Like Synoviocytes.

Estell E, Silverstein A, Stefani R, Lee A, Murphy L, Shah R J Orthop Res. 2019; 37(9):1979-1987.

PMID: 31062877 PMC: 6834361. DOI: 10.1002/jor.24340.

A Functional Tissue-Engineered Synovium Model to Study Osteoarthritis Progression and Treatment.

Stefani R, Halder S, Estell E, Lee A, Silverstein A, Sobczak E Tissue Eng Part A. 2018; 25(7-8):538-553.

PMID: 30203722 PMC: 6482911. DOI: 10.1089/ten.TEA.2018.0142.

Toward understanding the role of cartilage particulates in synovial inflammation.

Silverstein A, Stefani R, Sobczak E, Tong E, Attur M, Shah R Osteoarthritis Cartilage. 2017; 25(8):1353-1361.

PMID: 28365462 PMC: 5554538. DOI: 10.1016/j.joca.2017.03.015.

Effect of interleukin-1 on hyaluronate synthesis by synovial fibroblastic cells.

Konttinen Y, Saari H, Nordstrom D Clin Rheumatol. 1991; 10(2):151-4.

PMID: 1914415 DOI: 10.1007/BF02207654.

Sodium hyaluronate increases vascular ingrowth in the rabbit ear chamber.

Lebel L, Gerdin B Int J Exp Pathol. 1991; 72(2):111-8.

PMID: 1707647 PMC: 2002300.

References

Chen T . In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977; 104(2):255-62. DOI: 10.1016/0014-4827(77)90089-1. View

Schumacher H, Kitridou R . Synovitis of recent onset. A clinicopathologic study during the first month of disease. Arthritis Rheum. 1972; 15(5):465-85. DOI: 10.1002/art.1780150502. View

Castor C . Regulation of connective tissue metabolism by autacoid mediators. J Rheumatol Suppl. 1983; 11:55-60. View

Golds E, Santer V, Killackey J, Roughley P . Mononuclear cell factors stimulate the concomitant secretion of distinct latent proteoglycan, gelatin and collagen degrading enzymes from human skin fibroblasts and synovial cells. J Rheumatol. 1983; 10(6):861-71. View

ZIFF M . Relation of cellular infiltration of rheumatoid synovial membrane to its immune response. Arthritis Rheum. 1974; 17(3):313-9. DOI: 10.1002/art.1780170317. View

Pulkki K, Vuorio E, Jalava S . The effect of rheumatoid synovial fluid macrophages on DNA, glycosaminoglycan and collagen synthesis by synovial fibroblasts. Rheumatol Int. 1983; 3(3):133-8. DOI: 10.1007/BF00541193. View

Castor C, Bignall M, Hossler P, Roberts D . Connective tissue activation. XXI. Regulation of glycosaminoglycan metabolism by lymphocyte (CTAP-I) and platelet (CTAP-III) growth factors. In Vitro. 1981; 17(9):777-85. DOI: 10.1007/BF02618444. View

de Jong M . Esterase staining of human peripheral blood mononuclear cells. J Immunol Methods. 1979; 26(2):193-6. DOI: 10.1016/0022-1759(79)90082-6. View

Vuorio E . Rheumatoid disease in cultured human synovial cells. A biochemical study on glycosaminoglycans, proteins and plasma membranes of synovial fibroblasts in culture. Scand J Clin Lab Invest Suppl. 1977; (149):1-72. View

10.

Saarni H, Tammi M . A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium. Anal Biochem. 1977; 81(1):40-6. DOI: 10.1016/0003-2697(77)90596-6. View

11.

Betts W, Cleland L . Effect of metal chelators and antiinflammatory drugs on the degradation of hyaluronic acid. Arthritis Rheum. 1982; 25(12):1469-76. DOI: 10.1002/art.1780251213. View

12.

Krane S . Heberden Oration 1980: aspects of the cell biology of the rheumatoid synovial lesion. Ann Rheum Dis. 1981; 40(5):433-48. PMC: 1000778. DOI: 10.1136/ard.40.5.433. View

13.

Clarris B, Hamilton J . Peripheral blood mononuclear cells stimulate N-acetyl-beta-glucosaminidase levels of human synovial fibroblast-like cells. Rheumatol Int. 1985; 5(2):55-60. DOI: 10.1007/BF00270297. View

14.

Vuorio E, EINOLA S, Hakkarainen S, Penttinen R . Synthesis of underpolymerized hyaluronic acid by fibroblasts cultured from rheumatoid and non-rheumatoid synovitis. Rheumatol Int. 1982; 2(3):97-102. DOI: 10.1007/BF00541160. View

15.

Castor C . Synovial cell activation induced by a polypeptide mediator. Ann N Y Acad Sci. 1975; 256:304-17. DOI: 10.1111/j.1749-6632.1975.tb36057.x. View

16.

Hamerman D, Wood D . Interleukin 1 enhances synovial cell hyaluronate synthesis. Proc Soc Exp Biol Med. 1984; 177(1):205-10. DOI: 10.3181/00379727-177-1-rc1. View

17.

Wood D, Ihrie E, Dinarello C, Cohen P . Isolation of an interleukin-1-like factor from human joint effusions. Arthritis Rheum. 1983; 26(8):975-83. DOI: 10.1002/art.1780260806. View

18.

Korn J, Torres D, DOWNIE E . Clonal heterogeneity in the fibroblast response to mononuclear cell derived mediators. Arthritis Rheum. 1984; 27(2):174-9. DOI: 10.1002/art.1780270208. View

19.

Hamilton J . Synovial cell activation. Studies on the mechanism of action of synovial activator activity. J Rheumatol. 1983; 10(6):872-80. View

20.

Clarris B, Fraser J, Muirden K, Malcolm L, Holmes M, Rogers K . Stimulation of glycosaminoglycan production and lysosomal activity of human synovial cells in culture by low environmental pH. Ann Rheum Dis. 1984; 43(2):313-9. PMC: 1001490. DOI: 10.1136/ard.43.2.313. View