Evidence for the Compartmentation of Pyruvate Metabolism in Perfused Rat Skeletal Muscle

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Dec 15

PMID 6667265

Citations 3

Authors

P Schadewaldt

U Munch

W STAIB

Affiliations

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Abstract

In rat hindlimbs perfused with [1-14C]pyruvate and 5 mM-dichloroacetate, the calculated apparent rate of pyruvate decarboxylation was decreased with increasing perfusate pyruvate concentrations. However, in the absence of dichloroacetate the apparent rate of decarboxylation increased under these conditions. Dichloroacetate enhanced [1-14C]pyruvate uptake, but decreased the specific radioactivity of effluent lactate. Glycogen metabolism remained unaffected. The results were not consistent with a common pyruvate pool, but provide evidence for the compartmentation of pyruvate metabolism.

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