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Activation of Pyruvate Dehydrogenase in Perfused Rat Heart by Dichloroacetate (Short Communication)

Overview
Journal Biochem J
Specialty Biochemistry
Date 1973 Jun 1
PMID 16742828
Citations 59
Authors
S Whitehouse
P J Randle
Affiliations
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Abstract

The activity of pyruvate dehydrogenase was assayed in extracts of rat hearts perfused in vitro with media containing glucose and insulin+/-acetate+/-dichloroacetate. Dichloroacetate (100mum, 1mm or 10mm) increased the activity of pyruvate dehydrogenase in perfusions with glucose or glucose+acetate. Evidence is given that dichloroacetate may facilitate the conversion of pyruvate dehydrogenase from an inactive (phosphorylated) form into an active (dephosphorylated) form.

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