Abnormal Secretion of Proteins into Bile from Colchicine-treated Isolated Perfused Rat Livers

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Nov 15

PMID 6661206

Citations 10

Authors

S G Barnwell

R Coleman

Affiliations

Soon will be listed here.

Abstract

The microtubule poison, colchicine, caused an abnormal output of a variety of proteins into rat bile. After 3 h of exposure to the drug, livers were isolated and perfused with media of defined protein composition. There was no essential change in permeability of the hepatobiliary system to proteins (e.g. bovine serum albumin) entering bile from the perfusion fluid. The rat (serum) albumin and fibrinogen that were secreted into bile from colchicine-treated livers were probably derived from the hepatocytes. Disruption of the microtubular system reduces the secretion of proteins at the sinusoidal face of the hepatocyte and results in an accumulation of secretory vesicles in the cytoplasm. It is suggested that under these conditions some of the vesicles discharge their contents into the bile canaliculus.

Citing Articles

The effects of colchicine on secretion into bile of bile salts, phospholipids, cholesterol and plasma membrane enzymes: bile salts are secreted unaccompanied by phospholipids and cholesterol.

Barnwell S, Lowe P, Coleman R Biochem J. 1984; 220(3):723-31.

PMID: 6466298 PMC: 1153689. DOI: 10.1042/bj2200723.

Transcytosis and paracellular movements of horseradish peroxidase across liver parenchymal tissue from blood to bile. Effects of alpha-naphthylisothiocyanate and colchicine.

Lowe P, Kan K, Barnwell S, Sharma R, Coleman R Biochem J. 1985; 229(2):529-37.

PMID: 4038279 PMC: 1145087. DOI: 10.1042/bj2290529.

1-Naphthylisothiocyanate-induced permeability of hepatic tight junctions to proteins.

Kan K, Coleman R Biochem J. 1986; 238(2):323-8.

PMID: 3800941 PMC: 1147138. DOI: 10.1042/bj2380323.

The effect of colchicine on the development of lithocholic acid-induced cholestasis. A study of the role of microtubules in intracellular cholesterol transport.

Barnwell S, Yousef I, Tuchweber B Biochem J. 1986; 236(2):345-50.

PMID: 3753453 PMC: 1146846. DOI: 10.1042/bj2360345.

Uptake of low density lipoproteins by rat tissues. Special emphasis on the luteinized ovary.

REAVEN E, Chen Y, Spicher M, Hwang S, MONDON C, Azhar S J Clin Invest. 1986; 77(6):1971-84.

PMID: 3711341 PMC: 370558. DOI: 10.1172/JCI112526.

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