Characterization of a Human Genomic DNA Fragment Coding for a Myosin Heavy Chain

Overview

Journal Hum Genet

Specialty Genetics

Date 1983 Jan 1

PMID 6654335

Citations 2

Authors

H Appelhans

H P Vosberg

Affiliations

Soon will be listed here.

Abstract

A DNA segment from the human genome with information for myosin heavy chain (MHC) was isolated from a human genomic DNA library cloned in lambda Charon 4A phages. The isolation was accomplished by a myosin cDNA probe obtained from rabbit heart muscle mRNA (Sinha et al. 1982). The selected human DNA clone, designated lambda gMHC1, contains a genomic DNA fragment of about 14 kilobase pairs. The transcriptional polarity of this DNA was determined. The 5'-end of the gene is missing from the cloned fragment. This human gene exhibits sequence homology to MHC DNA of rabbit and chicken, but not to an MHC sequence of nematode. The isolated gene fragment is a member of the human MHC multi-gene family, which is presumed to consist of probably more than ten separate sarcomeric MHC genes per haploid genome.

Citing Articles

Chromosomal localization of a human myosin heavy-chain gene by in situ hybridization.

Rappold G, Vosberg H Hum Genet. 1983; 65(2):195-7.

PMID: 6654334 DOI: 10.1007/BF00286663.

Isolation and characterization of the complete human beta-myosin heavy chain gene.

DIEDERICH K, Eisele I, Ried T, Jaenicke T, Lichter P, Vosberg H Hum Genet. 1989; 81(3):214-20.

PMID: 2522082 DOI: 10.1007/BF00278991.

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