Dot-blotting--a Novel Screening Assay for Antibodies in Hybridoma Cultures

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1983 Nov 11

PMID 6644033

Citations 8

Authors

J Sternberg

P Jeppesen

Affiliations

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Abstract

A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.

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