Light Scattering Measurements Supporting Helical Structures for Chromatin in Solution

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 May 1

PMID 662693

Citations 17

Authors

A M Campbell

R I Cotter

J F Pardon

Affiliations

Soon will be listed here.

Abstract

Laser light scattering measurements have been made on a series of polynucleosomes containing from 50 to 150 nucleosomes. Radii of gyration have been determined as a function of polynucleosome length for different ionic strength solutions. The results suggest that at low ionic strength the chromatin adopts a loosely helical structure rather than a random coil. The helix becomes more regular on increasing the ionic strength, the dimension resembling those proposed by Finch and Klug for their solenoid model.

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