Isolation and Partial Characterization of a Lectin from Euphorbia Heterophylla Seeds

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Oct 1

PMID 6626171

Citations 1

Authors

M Nsimba-Lubaki

W J Peumans

A R Carlier

Affiliations

Soon will be listed here.

Abstract

An N-acetylgalactosamine-specific lectin was isolated from Euphorbia heterophylla seeds by affinity chromatography on cross-linked arabinogalactan. It is a dimeric protein of two identical subunits of Mr 32 000, and differs structurally from all previously known Euphorbiaceae lectins. Its distribution over the seed is typical in that it is merely confined to the primary axes.

Citing Articles

Lectins in castor bean seedlings.

Harley S, Beevers H Plant Physiol. 1986; 80(1):1-6.

PMID: 16664561 PMC: 1075046. DOI: 10.1104/pp.80.1.1.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Olsnes S . Ricin and ricinus agglutinin, toxic lectins from castor bean. Methods Enzymol. 1978; 50:330-5. DOI: 10.1016/0076-6879(78)50037-2. View

Kilpatrick D, Jeffree C, Lockhart C, Yeoman M . Immunological evidence for structural similarity among lectins from species of the Solanaceae. FEBS Lett. 1980; 113(1):129-33. DOI: 10.1016/0014-5793(80)80511-4. View

Peumans W, Stinissen H, Carlier A . Isolation and partial characterization of wheat-germ-agglutinin-like lectins from rye (Secale cereale) and barley (Hordeum vulgare) embryos. Biochem J. 1982; 203(1):239-43. PMC: 1158215. DOI: 10.1042/bj2030239. View

Tully R, Beevers H . Protein bodies of castor bean endosperm: isolation, fractionation, and the characterization of protein components. Plant Physiol. 1976; 58(6):710-6. PMC: 542292. DOI: 10.1104/pp.58.6.710. View

Falasca A, Franceschi C, Rossi C, Stirpe F . Mitogenic and haemagglutinating properties of a lectinpurified from Hura crepitans seeds. Biochim Biophys Acta. 1980; 632(1):95-105. DOI: 10.1016/0304-4165(80)90252-4. View

Goldstein I, Hayes C . The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem. 1978; 35:127-340. DOI: 10.1016/s0065-2318(08)60220-6. View

Barbieri L, Lorenzoni E, Stirpe F . Inhibition of protein synthesis in vitro by a lectin from Momordica charantia and by other haemagglutinins. Biochem J. 1979; 182(2):633-5. PMC: 1161349. DOI: 10.1042/bj1820633. View

Hankins C, Kindinger J, Shannon L . Legume Lectins: I. Immunological Cross-Reactions between the Enzymic Lectin from Mung Beans and other Well Characterized Legume Lectins. Plant Physiol. 1979; 64(1):104-7. PMC: 543033. DOI: 10.1104/pp.64.1.104. View

10.

Peumans W, Carlier A, Delaey B . Preparation and Characterization of a Highly Active Cell-free Protein-synthesizing System from Dry Pea Primary Axes. Plant Physiol. 1980; 66(4):584-7. PMC: 440683. DOI: 10.1104/pp.66.4.584. View

11.

Gasperi-Campani A, Barbieri L, Lorenzoni E, Montanaro L, Sperti S, Bonetti E . Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro. Biochem J. 1978; 174(2):491-6. PMC: 1185939. DOI: 10.1042/bj1740491. View

12.

Olsnes S . Toxic and nontoxic lectins from Abrus precatorius. Methods Enzymol. 1978; 50:323-30. DOI: 10.1016/0076-6879(78)50036-0. View

13.

Youle R, Huang A . Protein Bodies from the Endosperm of Castor Bean: Subfractionation, Protein Components, Lectins, and Changes during Germination. Plant Physiol. 1976; 58(6):703-9. PMC: 542291. DOI: 10.1104/pp.58.6.703. View