Preparation and Characterization of a Highly Active Cell-free Protein-synthesizing System from Dry Pea Primary Axes

Overview

Journal Plant Physiol

Specialty Physiology

Date 1980 Oct 1

PMID 16661482

Citations 4

Authors

W J Peumans

A R Carlier

B M Delaey

Affiliations

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Abstract

A highly active and reliable cell-free protein-synthesizing system from dry pea primary axes has been developed. The system has been optimized with respect to both overall activity and translational fidelity. Under optimal conditions, the system incorporates nearly 1,000 picomoles leucine per 50 microliters incubation mixture. At limiting tobacco mosaic virus RNA concentrations, its efficiency exceeds 1,000 picomoles leucine per picomole template molecules. The maximal size of the polypeptides coded for by tobacco mosaic virus RNA goes up to 180,000. A procedure has been worked out to reduce the endogenous template activity of the system. the dramatic increase of the activity of this pea cell-free system as compared to previous attempts can be explained by the use of Cl(-)-free buffers.

Citing Articles

A lectin from Bryonia dioica root stocks.

Peumans W, Nsimba-Lubaki M, Carlier A, Van Driessche E Planta. 2013; 160(3):222-8.

PMID: 24258504 DOI: 10.1007/BF00402858.

In vitro synthesis of lectins in cell-free extracts from dry wheat and rye embryos.

Peumans W, Stinissen H, Tierens M, Carlier A Plant Cell Rep. 2013; 1(5):212-4.

PMID: 24257711 DOI: 10.1007/BF00270238.

Isolation and partial characterization of a lectin from Euphorbia heterophylla seeds.

Nsimba-Lubaki M, Peumans W, Carlier A Biochem J. 1983; 215(1):141-5.

PMID: 6626171 PMC: 1152373. DOI: 10.1042/bj2150141.

A lectin from elder (Sambucus nigra L.) bark.

Broekaert W, Nsimba-Lubaki M, Peeters B, Peumans W Biochem J. 1984; 221(1):163-9.

PMID: 6466312 PMC: 1144016. DOI: 10.1042/bj2210163.

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