Neurotrophic Effects of Hippocampal Extracts on Medial Septal Nucleus in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Apr 1

PMID 6585816

Citations 6

Authors

K Ojika

S H Appel

Affiliations

Soon will be listed here.

Abstract

Within peripheral sympathetic and sensory systems, target tissue provides diffusible factors such as nerve growth factor that influence neuronal survival, growth, and differentiation. To determine whether target tissue may exert retrograde effects within the central nervous system, we have used hippocampus as a source of neurotrophic factors and cultured medial septal nucleus explants as a source of neurons that may respond to such factors. Soluble extracts from rat hippocampus enhance cholinergic activity (choline acetyltransferase, choline uptake, acetylcholine synthesis) of the rat medial septal nucleus cultured in serum-free defined medium. The enhancement is dose dependent, relatively specific for hippocampus, and varies with age, reaching a peak in 2- to 3-week-old rat hippocampus. The enhancing activity of the hippocampal extract appears to be mediated by protease-sensitive polypeptide(s) that differ from nerve growth factor in biological and chemical characteristics.

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References

Yamamura H, Snyder S . Choline: high-affinity uptake by rat brain synaptosomes. Science. 1972; 178(4061):626-8. DOI: 10.1126/science.178.4061.626. View

Honegger P, Lenoir D . Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures. Brain Res. 1982; 255(2):229-38. DOI: 10.1016/0165-3806(82)90023-2. View

GODFREY E, Schrier B, Nelson P . Source and target cell specificities of a conditioned medium factor that increases choline acetyltransferase activity in cultured spinal cord cells. Dev Biol. 1980; 77(2):403-18. DOI: 10.1016/0012-1606(80)90484-4. View

Bayer S . The development of the septal region in the rat. I. Neurogenesis examined with 3H-thymidine autoradiography. J Comp Neurol. 1979; 183(1):89-106. DOI: 10.1002/cne.901830108. View

Cowan W, Wenger E . Degeneration in the nucleus of origin of the preganglionic fibers to the chick ciliary ganglion following early removal of the optic vesicle. J Exp Zool. 1968; 168(1):105-23. DOI: 10.1002/jez.1401680109. View

Johnson D, PILAR G . The release of acetylcholine from post-ganglionic cell bodies in response to depolarization. J Physiol. 1980; 299:605-19. PMC: 1279244. DOI: 10.1113/jphysiol.1980.sp013144. View

Crutcher K, Collins F . In vitro Evidence for two distinct hippocampal growth factors: basis of neuronal plasticity?. Science. 1982; 217(4554):67-8. DOI: 10.1126/science.7089542. View

Cohen S . PURIFICATION OF A NERVE-GROWTH PROMOTING PROTEIN FROM THE MOUSE SALIVARY GLAND AND ITS NEURO-CYTOTOXIC ANTISERUM. Proc Natl Acad Sci U S A. 1960; 46(3):302-11. PMC: 222831. DOI: 10.1073/pnas.46.3.302. View

Bjorklund A, Stenevi U . Regeneration of monoaminergic and cholinergic neurons in the mammalian central nervous system. Physiol Rev. 1979; 59(1):62-100. DOI: 10.1152/physrev.1979.59.1.62. View

10.

Segal M, Landis S . Afferents to the hippocampus of the rat studied with the method of retrograde transport of horseradish peroxidase. Brain Res. 1974; 78(1):1-15. DOI: 10.1016/0006-8993(74)90349-7. View

11.

Manthorpe M, Skaper S, Adler R, Landa K, Varon S . Cholinergic neuronotrophic factors: fractionation properties of an extract from selected chick embryonic eye tissues. J Neurochem. 1980; 34(1):69-75. DOI: 10.1111/j.1471-4159.1980.tb04622.x. View

12.

Nadler J, Matthews D, Cotman C, Lynch G . Development of cholinergic innervation in the hippocampal formation of the rat. II. Quantitative changes in choline acetyltransferase and acetylcholinesterase activities. Dev Biol. 1974; 36(1):142-54. DOI: 10.1016/0012-1606(74)90197-3. View

13.

Matthews D, Nadler J, Lynch G, Cotman C . Development of cholinergic innervation in the hippocampal formation of the rat. I. Histochemical demonstration of acetylcholinesterase activity. Dev Biol. 1974; 36(1):130-41. DOI: 10.1016/0012-1606(74)90196-1. View

14.

Appel S . A unifying hypothesis for the cause of amyotrophic lateral sclerosis, parkinsonism, and Alzheimer disease. Ann Neurol. 1981; 10(6):499-505. DOI: 10.1002/ana.410100602. View

15.

Kuhar M, Sethy V, Roth R, Aghajanian G . Choline: selective accumulation by central cholinergic neurons. J Neurochem. 1973; 20(2):581-93. DOI: 10.1111/j.1471-4159.1973.tb12157.x. View

16.

Schonfeld A, Thal L, Horowitz S, Katzman R . Heart conditioned medium promotes central cholinergic regeneration in vivo. Brain Res. 1981; 229(2):541-6. DOI: 10.1016/0006-8993(81)91019-2. View

17.

Hollyday M, Hamburger V . Reduction of the naturally occurring motor neuron loss by enlargement of the periphery. J Comp Neurol. 1976; 170(3):311-20. DOI: 10.1002/cne.901700304. View

18.

Fonnum F . Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities. Biochem J. 1969; 115(3):465-72. PMC: 1185125. DOI: 10.1042/bj1150465. View

19.

Sperry R . CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS. Proc Natl Acad Sci U S A. 1963; 50:703-10. PMC: 221249. DOI: 10.1073/pnas.50.4.703. View

20.

Giller Jr E, Schrier B, Shainberg A, Fisk H, Nelson P . Choline acetyltransferase activity is increased in combined cultures of spinal cord and muscle cells from mice. Science. 1973; 182(4112):588-9. DOI: 10.1126/science.182.4112.588. View