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Utilization of Plasmid PULB113 (RP4::mini-Mu) to Construct a Linkage Map of Erwinia Carotovora Subsp. Chrysanthemi

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1983 Jun 1
PMID 6574127
Citations 16
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Abstract

We report experimental evidence that pULB113, an RP4::mini-Mu plasmid, mediates chromosome transfer in a strain of Erwinia carotovora subsp. chrysanthemi which does not accept the F episome. This allowed us to construct a genetic map of that strain by measuring the frequencies of cotransfer of different markers (thy, leu, pro, [his, trp], thyA, rpsL, ile).

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