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Mapping of Pgi and Gpd Genes Involved in C-1 Assimilation in the Obligate Methylotroph Methylobacillus Flagellatum

Overview
Journal Arch Microbiol
Specialty Microbiology
Date 1990 Jan 1
PMID 2105704
Citations 2
Authors
L V Kletsova
Y D Tsygankov
Affiliations
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Abstract

Homologous matings with plasmids R68.45 and pULB113, and also with Hfr type donor were employed for mapping pgi and gpd genes involved in C-1 metabolism in the obligate methylotroph Methylobacillus flagellatum. A preliminary map of the late chromosomal region was constructed on the basis of these experimental results. The C-1 markers were linked to methionine and leucine auxotrophy and nalidixic acid resistance markers. The phenomenon of retrotransfer, or shuttle transfer of chromosomal markers by Inc P1 plasmids, revealed earlier, was demonstrated for M. flagellatum.

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