A Comparison of Adenine and Some Derivatives on Pig Isolated Tracheal Muscle

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1983 Dec 1

PMID 6571222

Authors

W E Holden

A F Junod

Affiliations

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Abstract

We studied the muscle relaxation induced by adenine and several adenine derivatives in strips of tracheal smooth muscle from pigs; in addition their metabolism by the tissue was examined. Adenine relaxed tissue which was contracted by carbachol, histamine, or KCl. Adenine's potency was similar to that of adenosine and ATP (threshold about 4 X 10(-5)M). In tissues with carbachol-induced tone, the adenine effect differed from adenosine and ATP by being slower in onset and in 'washout' time. Furthermore, neither dipyridamole nor theophylline modified the response to adenine. The relationship was examined between pharmacological effects and the metabolism of [3H]-adenosine and [3H]-adenine. Both substrates were taken up by the tissue and converted to nucleotides, but relaxation correlated with nucleotide accumulation only in the case of [3H]-adenine. We conclude that the site and mechanism of adenine-induced relaxation is different from that of adenosine and ATP in porcine tracheal muscle.

References

Stanley P, Williams S . Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem. 1969; 29(3):381-92. DOI: 10.1016/0003-2697(69)90323-6. View

Randerath K, RANDERATH E . ION-EXCHANGE CHROMATOGRAPHY OF NUCLEOTIDES ON POLY-(ETHYLENEIMINE)-CELLULOSE THIN LAYERS. J Chromatogr. 1964; 16:111-25. DOI: 10.1016/s0021-9673(01)82445-6. View

Dobson Jr J, Rubio R, BERNE R . Role of adenine nucleotides, adenosine, and inorganic phosphate in the regulation of skeletal muscle blood flow. Circ Res. 1971; 29(4):375-84. DOI: 10.1161/01.res.29.4.375. View

FLEMING W, Westfall D, de la Lande I, Jellett L . Log-normal distribution of equiefective doses of norepinephrine and acetylcholine in several tissues. J Pharmacol Exp Ther. 1972; 181(2):339-45. View

Mentzer Jr R, Rubio R, BERNE R . Release of adenosine by hypoxic canine lung tissue and its possible role in pulmonary circulation. Am J Physiol. 1975; 229(6):1625-31. DOI: 10.1152/ajplegacy.1975.229.6.1625. View

Webb R, Bhalla R . Calcium sequestration by subcellular fractions isolated from vascular smooth muscle: effect of cyclic nucleotides and prostaglandins. J Mol Cell Cardiol. 1976; 8(2):145-57. DOI: 10.1016/0022-2828(76)90026-2. View

Coleman R . Effects of some purine derivatives on the guinea-pig trachea and their interaction with drugs that block adenosine uptake. Br J Pharmacol. 1976; 57(1):51-7. PMC: 1667005. DOI: 10.1111/j.1476-5381.1976.tb07655.x. View

Spedding M, WEETMAN D . Identification of separate receptors for adenosine and adenosine 5'-triphosphate in causing relaxations of the isolated taenia of the guinea-pig caecum. Br J Pharmacol. 1976; 57(2):305-10. PMC: 1667108. DOI: 10.1111/j.1476-5381.1976.tb07480.x. View

Rapaport E, Zamecnik P . Incorporation of adenosine into ATP: formation of compartmentalized ATP. Proc Natl Acad Sci U S A. 1976; 73(9):3122-5. PMC: 430951. DOI: 10.1073/pnas.73.9.3122. View

10.

Farmer J, Farrar D . Pharmacological studies with adenine, adenosine and some phosphorylated derivatives on guinea-pig tracheal muscle. J Pharm Pharmacol. 1976; 28(10):748-52. DOI: 10.1111/j.2042-7158.1976.tb04040.x. View

11.

Clyman R, Manganiello V, Vaughan M . Calcium uptake by subcellular fractions of human umbilical artery. Am J Physiol. 1976; 231(4):1074-81. DOI: 10.1152/ajplegacy.1976.231.4.1074. View

12.

Schrader J, Gerlach E . Compartmentation of cardiac adenine nucleotides and formation of adenosine. Pflugers Arch. 1976; 367(2):129-35. DOI: 10.1007/BF00585148. View

13.

DE VERDIER C, Ericson A, Niklasson F, Westman M . Adenine metabolism in man. 1. After intravenous and peroral administration. Scand J Clin Lab Invest. 1977; 37(7):567-75. DOI: 10.3109/00365517709100648. View

14.

Dieterle Y, Ody C, Ehrensberger A, Stalder H, Junod A . Metabolism and uptake of adenosine triphosphate and adenosine by porcine aortic and pulmonary endothelial cells and fibroblasts in culture. Circ Res. 1978; 42(6):869-76. DOI: 10.1161/01.res.42.6.869. View

15.

Marz R, Wohlhueter R, Plagemann P . Purine and pyrimidine transport and phosphoribosylation and their interaction in overall uptake by cultured mammalian cells. A re-evaluation. J Biol Chem. 1979; 254(7):2329-38. View

16.

Akintonwa A, AUDITORE J, Green L . The toxicological effects of pure dietary adenine base in the rat. Toxicol Appl Pharmacol. 1979; 47(2):229-35. DOI: 10.1016/0041-008x(79)90316-8. View

17.

Lum C, Marz R, Plagemann P, Wohlhueter R . Adenosine transport and metabolism in mouse leukemia cells and in canine thymocytes and peripheral blood leukocytes. J Cell Physiol. 1979; 101(2):173-200. DOI: 10.1002/jcp.1041010202. View

18.

Christie J, Satchell D . Purine receptors in the trachea: is there a receptor for ATP?. Br J Pharmacol. 1980; 70(4):512-4. PMC: 2044384. DOI: 10.1111/j.1476-5381.1980.tb09768.x. View

19.

Jones T, Lefcoe N, Hamilton J . Pharmacological study of adenosine and related compounds on isolated guinea pig trachea: evidence for more than one type of purine receptor. Can J Physiol Pharmacol. 1980; 58(11):1356-65. DOI: 10.1139/y80-205. View

20.

Burnstock G . Review lecture. Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol. 1981; 313:1-35. PMC: 1274434. DOI: 10.1113/jphysiol.1981.sp013648. View