Thiamine Pyrophosphate Determination in Whole Blood and Erythrocytes by High Performance Liquid Chromatography

Overview

Journal Int J Vitam Nutr Res

Specialty Nutritional Sciences

Date 1984 Jan 1

PMID 6500839

Citations 7

Authors

A Floridi

M Pupita

C A Palmerini

C Fini

A Alberti Fidanza

Affiliations

Soon will be listed here.

Abstract

A sensitive method for the measurement of thiamine pyrophosphate in whole blood and erythrocytes, using a straight-phase high performance liquid chromatography (HPLC), is described. The method was based on sample extraction with trichloracetic acid, conversion of the vitamer to the corresponding high fluorescent thiochrome ester, and separation and measurement of the derivative by HPLC. Evidence for reproducibility and accuracy of the assay is presented. Data indicating the thiamine pyrophosphate levels to be expected in blood and erythrocytes of healthy humans are included.

Citing Articles

Assessment of Erythrocyte Transketolase, Whole Blood Thiamine Diphosphate, and Human Milk Thiamine Concentrations to Identify Infants and Young Children Responding Favorably to Therapeutic Thiamine Administration: Findings from the Lao Thiamine....

Hess S, Smith T, Arnold C, Jones K, Hampel D, Hiffler L Curr Dev Nutr. 2024; 8(6):103786.

PMID: 38974350 PMC: 11225667. DOI: 10.1016/j.cdnut.2024.103786.

Thiamine dose response in human milk with supplementation among lactating women in Cambodia: study protocol for a double-blind, four-parallel arm randomised controlled trial.

Whitfield K, Kroeun H, Green T, Wieringa F, Borath M, Sophonneary P BMJ Open. 2019; 9(7):e029255.

PMID: 31292183 PMC: 6624064. DOI: 10.1136/bmjopen-2019-029255.

Thiamine deficiency disorders: diagnosis, prevalence, and a roadmap for global control programs.

Whitfield K, Bourassa M, Adamolekun B, Bergeron G, Bettendorff L, Brown K Ann N Y Acad Sci. 2018; 1430(1):3-43.

PMID: 30151974 PMC: 6392124. DOI: 10.1111/nyas.13919.

High prevalence of thiamine (vitamin B1) deficiency in early childhood among a nationally representative sample of Cambodian women of childbearing age and their children.

Whitfield K, Smith G, Chamnan C, Karakochuk C, Sophonneary P, Kuong K PLoS Negl Trop Dis. 2017; 11(9):e0005814.

PMID: 28873391 PMC: 5600402. DOI: 10.1371/journal.pntd.0005814.

Nutritional neuropathies.

Hammond N, Wang Y, Dimachkie M, Barohn R Neurol Clin. 2013; 31(2):477-89.

PMID: 23642720 PMC: 4199287. DOI: 10.1016/j.ncl.2013.02.002.