Characterisation of a Lambda Transducing Phage Carrying the F Conjugation Gene TraG

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 6446656

Citations 6

Authors

N Willetts

J Maule

Affiliations

Soon will be listed here.

Abstract

A lambda transducing phage carrying the traGSTD genes of the E. coli K12 factor F was isolated by an in vivo technique, and characterized in tra complementation tests, by determining its restriction endonuclease fragment sizes, and by measuring heteroduplex molecules. The size and location on the F physical map of the tra transducing segment was thereby determined. Comparison of the proteins synthesized in UV-irradiated cells by this phage and by a derivative carrying the amber traG79 mutation, allowed the traG product to be identified as a protein of molecular weight 100,000. In the same experiments, the sizes of the traT and traD products made by the phage were also measured, being 25,000 and 85,000 daltons respectively.

Citing Articles

traG protein of the F sex factor of Escherichia coli K-12 and its role in conjugation.

Manning P, Morelli G, Achtman M Proc Natl Acad Sci U S A. 1981; 78(12):7487-91.

PMID: 7038680 PMC: 349293. DOI: 10.1073/pnas.78.12.7487.

Promoter-distal region of the tra operon of F-like sex factor R100 in Escherichia coli K-12.

Hansen B, Manning P, Achtman M J Bacteriol. 1982; 150(1):89-99.

PMID: 6277874 PMC: 220085. DOI: 10.1128/jb.150.1.89-99.1982.

Overproduction, purification and characterization of the F traT protein.

Minkley Jr E, Willetts N Mol Gen Genet. 1984; 196(2):225-35.

PMID: 6238226 DOI: 10.1007/BF00328054.

The effect of tra mutations on the synthesis of the F-pilin membrane polypeptide.

Moore D, Sowa B Mol Gen Genet. 1981; 184(2):260-4.

PMID: 6120442 DOI: 10.1007/BF00272914.

Nucleotide sequence of the surface exclusion genes traS and traT from the IncF0 lac plasmid pED208.

Finlay B, Paranchych W J Bacteriol. 1986; 166(3):713-21.

PMID: 3011738 PMC: 215180. DOI: 10.1128/jb.166.3.713-721.1986.

References

Linn T, Goman M, Scaife J . Studies on the control of the genes for transcription and translation in Escherichia coli K12 I. tufB and rplA, K have separate promoters. J Mol Biol. 1979; 130(4):405-20. DOI: 10.1016/0022-2836(79)90431-5. View

Achtman M, Willetts N . Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons. J Bacteriol. 1972; 110(3):857-63. PMC: 247503. DOI: 10.1128/jb.110.3.857-863.1972. View

Achtman M, Willetts N, Clark A . Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972; 110(3):831-42. PMC: 247500. DOI: 10.1128/jb.110.3.831-842.1972. View

Enquist L, WEISBERG R . The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda. Virology. 1976; 72(1):147-53. DOI: 10.1016/0042-6822(76)90319-6. View

Willetts N, McIntire S . Isolation and characterisation of lambdatra transducing phages from EDFL223 (Flac traB::EDlambda4). J Mol Biol. 1978; 126(3):525-49. DOI: 10.1016/0022-2836(78)90057-8. View

Novotny C, Fives-Taylor P . Retraction of F pili. J Bacteriol. 1974; 117(3):1306-11. PMC: 246614. DOI: 10.1128/jb.117.3.1306-1311.1974. View

Otsubo E, Nishimura Y, Hirota Y . Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis. Genetics. 1970; 64(2):173-88. PMC: 1212394. DOI: 10.1093/genetics/64.2.173. View

Willetts N, Maule J . Investigations of the F conjugation gene traI:traI mutants and lambdatraI transducing phages. Mol Gen Genet. 1979; 169(3):325-36. DOI: 10.1007/BF00382278. View

Blattner F, Fiandt M, Hass K, Twose P, SZYBALSKI W . Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance. Virology. 1974; 62(2):458-71. DOI: 10.1016/0042-6822(74)90407-3. View

10.

Willetts N . Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12. J Bacteriol. 1974; 118(3):778-82. PMC: 246821. DOI: 10.1128/jb.118.3.778-782.1974. View

11.

Everett R, Willetts N . Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F. J Mol Biol. 1980; 136(2):129-50. DOI: 10.1016/0022-2836(80)90309-5. View

12.

Achtman M, Willetts N, Clark A . Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971; 106(2):529-38. PMC: 285127. DOI: 10.1128/jb.106.2.529-538.1971. View

13.

Achtman M, Skurray R, Thompson R, Helmuth R, Hall S, Beutin L . Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol. 1978; 133(3):1383-92. PMC: 222176. DOI: 10.1128/jb.133.3.1383-1392.1978. View

14.

McParland R, Brown L, Pearson G . Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens. J Virol. 1976; 19(3):1006-11. PMC: 354941. DOI: 10.1128/JVI.19.3.1006-1011.1976. View

15.

Landy A, Ross W . Viral integration and excision: structure of the lambda att sites. Science. 1977; 197(4309):1147-60. PMC: 1994661. DOI: 10.1126/science.331474. View

16.

Kingsman A, Willetts N . The requirements for conjugal DNA synthesis in the donor strain during flac transfer. J Mol Biol. 1978; 122(3):287-300. DOI: 10.1016/0022-2836(78)90191-2. View

17.

Kennedy N, Beutin L, Achtman M, Skurray R, Rahmsdorf U, Herrlich P . Conjugation proteins encoded by the F sex factor. Nature. 1977; 270(5638):580-5. DOI: 10.1038/270580a0. View

18.

Haggerty D, Scheif R . Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H. J Virol. 1976; 18(2):659-63. PMC: 515593. DOI: 10.1128/JVI.18.2.659-663.1976. View

19.

McIntire S, Willetts N . Transfer-deficient cointegrates of Flac and lambda prophage. Mol Gen Genet. 1980; 178(1):165-72. DOI: 10.1007/BF00267225. View

20.

Willetts N, Johnson G . R.SmaI cleavage map of the transfer region of the E. coli K12 sex factor F. Genet Res. 1979; 34(2):195-202. DOI: 10.1017/s001667230001942x. View