Transfer-deficient Cointegrates of Flac and Lambda Prophage

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Apr 1

PMID 6446018

Citations 8

Authors

S McIntire

N Willetts

Affiliations

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Abstract

Twelve transfer-deficient mutants of the plasmid Flac were obtained by insertion of prophage lambda into secondary attachment sites within the transfer region. Insertions into eight different tra genes were identified. These mutations were strongly polar on expression of tra genes previously mapped "downstream", and thus confirmed that the genes traA through traD form a single operon. However, some continued expression of traI suggested that this was transcribed in part from a promoter located between traD and traI, and in part from the transfer operon promoter. One insertion early in the transfer operon produced a plasmid-specific tra mutation not complemented by R100-1 or R1-19: this insertion was into a new gene (traY), located before traA as the first member of the transfer operon. Partial tra deletion mutants were obtained as 42 degrees C--survivors from several of the Flac tra::ED lambda 4 plasmids, and their properties are described.

Citing Articles

Transfer protein TraY of plasmid R1 stimulates TraI-catalyzed oriT cleavage in vivo.

Karl W, Bamberger M, Zechner E J Bacteriol. 2001; 183(3):909-14.

PMID: 11208788 PMC: 94957. DOI: 10.1128/JB.183.3.909-914.2001.

Characterization of the Escherichia coli F factor traY gene product and its binding sites.

Nelson W, MORTON B, Lahue E, Matson S J Bacteriol. 1993; 175(8):2221-8.

PMID: 8468282 PMC: 204507. DOI: 10.1128/jb.175.8.2221-2228.1993.

sfrA and sfrB products of Escherichia coli K-12 are transcriptional control factors.

Beutin L, Manning P, Achtman M, Willetts N J Bacteriol. 1981; 145(2):840-4.

PMID: 7007352 PMC: 217187. DOI: 10.1128/jb.145.2.840-844.1981.

Characterisation of a lambda transducing phage carrying the F conjugation gene traG.

Willetts N, Maule J Mol Gen Genet. 1980; 178(3):675-80.

PMID: 6446656 DOI: 10.1007/BF00337878.

Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.

Thompson R, Taylor L Mol Gen Genet. 1982; 188(3):513-8.

PMID: 6298579 DOI: 10.1007/BF00330058.

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