Molecular Cloning of Unintegrated Moloney Mouse Sarcoma Virus DNA in Bacteriophage Lambda

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 Apr 1

PMID 6445561

Citations 22

Authors

I M Verma

M H Lai

R A Bosselman

M A McKennett

H Fan

A Berns

Affiliations

Soon will be listed here.

Abstract

The covalently closed circular forms of unintegrated viral DNA obtained from cells infected with Moloney mouse sarcoma virus was cloned in bacteriophage lambda. The viral DNA was cleaved with restriction endonuclease HindIII and inserted in the unique HindIII site of lambda Charon 21A DNA. Recombinant clones containing virus-reactive DNA sequences were analyzed by restriction endonuclease mapping, R-loop formation, and infectivity assays. Two of eight genome-length recombinant clones characterized contained the large terminal repeat. Only the recombinant clones containing the large terminal repeat were able to induce focus formation in uninfected mouse fibroblasts.

Citing Articles

Biogenesis of a transforming gene.

Van Beveren C, Goddard J, Berns A, Verma I Mol Biol Rep. 1981; 7(1-3):163-5.

PMID: 7254205 DOI: 10.1007/BF00778748.

Analysis of the env gene of a molecularly cloned and biologically active Moloney mink cell focus-forming proviral DNA.

Bosselman R, van Straaten F, Van Beveren C, Verma I, Vogt M J Virol. 1982; 44(1):19-31.

PMID: 7143566 PMC: 256237. DOI: 10.1128/JVI.44.1.19-31.1982.

Demonstration of biological activity and nucleotide sequence of an in vitro synthesized clone of the Moloney murine sarcoma virus mos gene.

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Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants.

Kollek R, Stocking C, Smadja-Joffe F, Ostertag W J Virol. 1984; 50(3):717-24.

PMID: 6328001 PMC: 255729. DOI: 10.1128/JVI.50.3.717-724.1984.

Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.

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