Arachidonic Acid and Prostaglandin Endoperoxide Metabolism in Isolated Rabbit and Coronary Microvessels and Isolated and Cultivated Coronary Microvessel Endothelial Cells

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1983 Nov 1

PMID 6415116

Citations 23

Authors

M E Gerritsen

C D Cheli

Affiliations

Soon will be listed here.

Abstract

Isolated microvessels and isolated and cultured microvessel endothelial cells were prepared from rabbit cardiac muscle. Pathways of arachidonic acid metabolism were determined by measurement of exogenous substrate utilization [( 1-14C]arachidonic acid incorporation and release from intact tissue and cells; [1-14C]prostaglandin H2 (PGH2) metabolism by broken cell preparations) and by quantification of endogenous products (immunoreactive 6-keto-prostaglandin F1 alpha (PGF1 alpha) and prostaglandin E (PGE) release) by selective radioimmunoassay. Rabbit coronary microvessels and derived microvascular endothelial cells (RCME cells) synthesized two major products of the cyclooxygenase pathway: 6-keto-PGF1 alpha (hydrolytic product of prostaglandin I2) and PGE2. A reduced glutathione requiring PGH-E isomerase was demonstrated in coronary microvessels and RCME cells, but not in rabbit circumflex coronary artery or aorta. In addition, a minor amount of a compound exhibiting similar characteristics to 6-keto-PGE1 was found to be produced by microvessels and RCME cells. Measurement of endogenously released prostaglandins indicated that under basal and stimulated conditions, PGE release exceeded that of 6-keto-PGF1 alpha. Microvessels and microvessel endothelial cells derived from cardiac muscle of rabbit exhibit pathways of arachidonate metabolism that are different from those of many large blood vessels and derived endothelial cells.

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References

Hamberg M, Samuelsson B . On the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxidase. J Biol Chem. 1967; 242(22):5329-35. View

Allen J, Rasmussen H . Human red blood cells: prostaglandin E2, epinephrine, and isoproterenol alter deformability. Science. 1971; 174(4008):512-4. DOI: 10.1126/science.174.4008.512. View

BRODY M, Kadowitz P . Prostaglandins as modulators of the autonomic nervous system. Fed Proc. 1974; 33(1):48-60. View

Hensby C . Reduction of prostaglandin E2 to prostaglandin F2alpha by an enzyme in sheep blood. Biochim Biophys Acta. 1974; 348(1):145-54. DOI: 10.1016/0005-2760(74)90100-3. View

Messina E, Weiner R, Kaley G . Microcirculatory effects of prostaglandins E1, E2, and A1 in the rat mesentery and cremaster muscle. Microvasc Res. 1974; 8(1):77-89. DOI: 10.1016/0026-2862(74)90066-1. View

Messina E, Weiner R, Kaley G . Inhibition of bradykinin vasodilation and potentiation of norepinephrine and angiotensin vasoconstriction by inhibitors of prostaglandin synthesis in skeletal muscle of the rat. Circ Res. 1975; 37(4):430-7. DOI: 10.1161/01.res.37.4.430. View

Wagner R, Matthews M . The isolation and culture of capillary endothelium from epididymal fat. Microvasc Res. 1975; 10(3):286-97. DOI: 10.1016/0026-2862(75)90033-3. View

Brendel K, MEEZAN E, CARLSON E . Isolated brain microvessels: a purified, metabolically active preparation from bovine cerebral cortex. Science. 1974; 185(4155):953-5. DOI: 10.1126/science.185.4155.953. View

Messina E, Weiner R, Kaley G . Prostaglandins and local circulatory control. Fed Proc. 1976; 35(12):2367-75. View

10.

Moncada S, Gryglewski R, Bunting S, Vane J . A lipid peroxide inhibits the enzyme in blood vessel microsomes that generates from prostaglandin endoperoxides the substance (prostaglandin X) which prevents platelet aggregation. Prostaglandins. 1976; 12(5):715-37. DOI: 10.1016/0090-6980(76)90048-4. View

11.

Isakson P, Raz A, NEEDLEMAN P . Selective incorporation of 14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to 14C-prostaglandins. Prostaglandins. 1976; 12(5):739-48. DOI: 10.1016/0090-6980(76)90049-6. View

12.

Bunting S, Gryglewski R, Moncada S, Vane J . Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac ateries and inhibits platelet aggregation. Prostaglandins. 1976; 12(6):897-913. DOI: 10.1016/0090-6980(76)90125-8. View

13.

Ogino N, Miyamoto T, Yamamoto S, HAYAISHI O . Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme. J Biol Chem. 1977; 252(3):890-5. View

14.

Tai H, Yuan B . A simple and sensitive assay for 9-hydroxyprostaglandin dehydrogenase. Anal Biochem. 1977; 78(2):410-22. DOI: 10.1016/0003-2697(77)90102-6. View

15.

Hedqvist P . Basic mechanisms of prostaglandin action on autonomic neurotransmission. Annu Rev Pharmacol Toxicol. 1977; 17:259-79. DOI: 10.1146/annurev.pa.17.040177.001355. View

16.

Weksler B, Marcus A, Jaffe E . Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells. Proc Natl Acad Sci U S A. 1977; 74(9):3922-6. PMC: 431786. DOI: 10.1073/pnas.74.9.3922. View

17.

Moncada S, Herman A, Higgs E, Vane J . Differential formation of prostacyclin (PGX or PGI2) by layers of the arterial wall. An explanation for the anti-thrombotic properties of vascular endothelium. Thromb Res. 1977; 11(3):323-44. DOI: 10.1016/0049-3848(77)90185-2. View

18.

De Deckere E, Nugteren D, Ten Hoor F . Prostacyclin is the major prostaglandin released from the isolated perfused rabbit and rat heart. Nature. 1977; 268(5616):160-3. DOI: 10.1038/268160a0. View

19.

Omini C, Moncada S, Vane J . The effects of prostacyclin (PGI2) on tissues which detect prostaglandins (PG'S). Prostaglandins. 1977; 14(4):625-32. DOI: 10.1016/0090-6980(77)90189-7. View

20.

NEEDLEMAN P, BRONSON S, Wyche A, Sivakoff M, Nicolaou K . Cardiac and renal prostaglandin I2. Biosynthesis and biological effects in isolated perfused rabbit tissues. J Clin Invest. 1978; 61(3):839-49. PMC: 372599. DOI: 10.1172/JCI108998. View