Hidden Electrophoretic Variation at the Xanthine Dehydrogenase Locus in a Natural Population of Drosophila Melanogaster

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1983 Jun 1

PMID 6407892

Citations 5

Authors

B A Buchanan

D L Johnson

Affiliations

Soon will be listed here.

Abstract

Sixty-two isochromosomal lines of D. melanogaster were screened for cryptic electrophoretic variation at the xanthine dehydrogenase (XDH) locus. Sequential polyacrylamide vertical slab gel electrophoresis was performed using four electrophoretic criteria. A total of 15 classes of electromorphs were revealed. D. melanogaster appears to exhibit as much polymorphism at this locus as other extensively studied Drosophila species.--No evidence for loci on the X or second chromosomes which modified XDH mobility was found. Six of the electromorphs were mapped to the Xdh (ry) structural locus. Eight of the remaining nine classes exhibited mobility variation consistent with structural variation at the Xdh locus. The final class exhibited aberrant patterns and is under further study.

Citing Articles

Male accessory gland secretory protein polymorphism in natural populations of Drosophila nasuta nasuta and Drosophila sulfurigaster neonasuta.

Ravi Ram K, Ramesh S J Genet. 2008; 86(3):217-24.

PMID: 18305341 DOI: 10.1007/s12041-007-0029-4.

Catalytic properties of alcohol dehydrogenase isozymes specified by duplicate genes in the diploid plant Stephanomeria exigua.

Roose M Biochem Genet. 1984; 22(7-8):631-43.

PMID: 6388561 DOI: 10.1007/BF00485849.

Alteration of the gene for xanthine dehydrogenase in Drosophila following treatment with wildtype DNA.

Germeraad S Mol Gen Genet. 1985; 199(1):89-94.

PMID: 3923301 DOI: 10.1007/BF00327515.

Historical effective size and the level of genetic diversity in Drosophila melanogaster and Drosophila pseudoobscura.

Choudhary M, Singh R Biochem Genet. 1987; 25(1-2):41-51.

PMID: 3107543 DOI: 10.1007/BF00498950.

Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.

Selander R, Caugant D, Ochman H, Musser J, Gilmour M, Whittam T Appl Environ Microbiol. 1986; 51(5):873-84.

PMID: 2425735 PMC: 238981. DOI: 10.1128/aem.51.5.873-884.1986.

References

Gelbart W, McCarron M, Chovnick A . Extension of the limits of the XDH structural element in Drosophila melanogaster. Genetics. 1976; 84(2):211-32. PMC: 1213573. DOI: 10.1093/genetics/84.2.211. View

Coyne J . Lack of genic similarity between two sibling species of drosophila as revealed by varied techniques. Genetics. 1976; 84(3):593-607. PMC: 1213597. DOI: 10.1093/genetics/84.3.593. View

Bewley G . Heat stability studies at the alpha-glycerophosphate dehydrogenase locus in populations of Drosophila melanogaster. Biochem Genet. 1978; 16(7-8):769-75. DOI: 10.1007/BF00484734. View

Macintyre R, OBrien S . Interacting gene-enzyme systems in Drosophila. Annu Rev Genet. 1976; 10:281-318. DOI: 10.1146/annurev.ge.10.120176.001433. View

Nei M, Fuerst P, Chakraborty R . Subunit molecular weight and genetic variability of proteins in natural populations. Proc Natl Acad Sci U S A. 1978; 75(7):3359-62. PMC: 392775. DOI: 10.1073/pnas.75.7.3359. View

Chovnick A, Schalet A, KERNAGHAN R, Krauss M . THE ROSY CISTRON IN DROSOPHILA MELANOGASTER: GENETIC FINE STRUCTURE ANALYSIS. Genetics. 1964; 50:1245-59. PMC: 1210731. DOI: 10.1093/genetics/50.6.1245. View

Lusis A, Breen G, Paigen K . Nongenetic heterogeneity of mouse beta-galactosidase. J Biol Chem. 1977; 252(13):4613-8. View

McCarron M, ODonnell J, Chovnick A, Bhullar B, Hewitt J, Candido E . Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1979; 91(2):275-93. PMC: 1216366. DOI: 10.1093/genetics/91.2.275. View

Chovnick A, Gelbart W, McCarron M, Osmond B . Organization of the rosy locus in Drosophila melanogaster: evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1976; 84(2):233-55. PMC: 1213574. DOI: 10.1093/genetics/84.2.233. View

10.

Prakash S, Lewontin R, Hubby J . A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central, marginal and isolated populations of Drosophila pseudoobscura. Genetics. 1969; 61(4):841-58. PMC: 1212247. DOI: 10.1093/genetics/61.4.841. View

11.

Ohta T . Role of very slightly deleterious mutations in molecular evolution and polymorphism. Theor Popul Biol. 1976; 10(3):254-75. DOI: 10.1016/0040-5809(76)90019-8. View

12.

McCarron M, Gelbart W, Chovnick A . Intracistronic mapping of electrophoretic sites in Drosophila melanogaster: fidelity of information transfer by gene conversion. Genetics. 1974; 76(2):289-99. PMC: 1213066. DOI: 10.1093/genetics/76.2.289. View

13.

Cochrane B, Richmond R . Studies of esterase 6 in Drosophila melanogaster. I. The genetics of a posttranslational modification. Biochem Genet. 1979; 17(1-2):167-83. DOI: 10.1007/BF00484483. View

14.

Coyne J, Felton A, Lewontin R . Extent of genetic variation at a highly polymorphic esterase locus in Drosophila pseudoobscura. Proc Natl Acad Sci U S A. 1978; 75(10):5090-3. PMC: 336269. DOI: 10.1073/pnas.75.10.5090. View

15.

Cobbs G . Polymorphism for dimerizing ability at the esterase-5 locus in Drosophila pseudoobscura. Genetics. 1976; 82(1):53-62. PMC: 1213445. DOI: 10.1093/genetics/82.1.53. View

16.

Ramshaw J, Coyne J, Lewontin R . The sensitivity of gel electrophoresis as a detector of genetic variation. Genetics. 1979; 93(4):1019-37. PMC: 1214113. DOI: 10.1093/genetics/93.4.1019. View

17.

Maruyama T, Nei M . Genetic variability maintained by mutation and overdominant selection in finite populations. Genetics. 1981; 98(2):441-59. PMC: 1214452. DOI: 10.1093/genetics/98.2.441. View

18.

Golubovsky M, Ivano Y, Green M . Genetic instability in Drosophila melanogaster: putative multiple insertion mutants at the singed bristle locus. Proc Natl Acad Sci U S A. 1977; 74(7):2973-5. PMC: 431368. DOI: 10.1073/pnas.74.7.2973. View

19.

Bernstein S, Throckmorton L, Hubby J . Still more genetic variability in natural populations. Proc Natl Acad Sci U S A. 1973; 70(12):3928-31. PMC: 427359. DOI: 10.1073/pnas.70.12.3928. View