Phagocytosis by Sheep Alveolar Macrophages: Relationship Between Opsonin Concentration and Light Emission in the Presence of Luminol

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1978 Mar 1

PMID 640735

Citations 7

Authors

R L Ziprin

Affiliations

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Abstract

The observations reported may be applied to determining the effects of various compounds, e.g., environmental pollutants and agricultural chemicals, upon the phagocytic activity of alveolar macrophages, and the method described will aid in detecting compounds which alter Fc receptor activity. A direct linear relationship existed between the concentration of antibody used to opsonize bacterial particles and the quantity of luminol-dependent light emitted by a population of sheep alveolar macrophages exposed to the opsonized particles. The relationship can be illustrated with a Lineweaver-Burk-style double-reciprocal plot. An analogy is suggested between the kinetics of enzyme substrate reactions and the interaction of antibody-coated particles with Fc receptors on cell membranes.

Citing Articles

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Menitove J, Frenzke M, Braem S, Aster R Klin Wochenschr. 1982; 60(11):567-9.

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Chemiluminescence response of equine alveolar macrophages during stimulation with latex beads, or IgG-opsonized sheep red blood cells.

Dyer R, Leid R Inflammation. 1983; 7(2):169-82.

PMID: 6862592 DOI: 10.1007/BF00917821.

The stimulation of superoxide anion production in guinea-pig peritoneal macrophages and neutrophils by phorbol myristate acetate, opsonized zymosan and IgG2-containing soluble immune complexes.

Baxter M, Leslie R, Reeves W Immunology. 1983; 48(4):657-65.

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Human bronchoalveolar lavage cells and luminol-dependent chemiluminescence.

Williams A, COLE P J Clin Pathol. 1981; 34(2):167-71.

PMID: 6262385 PMC: 1146445. DOI: 10.1136/jcp.34.2.167.

In vitro stimulation of alveolar macrophage metabolic activity by polystyrene in the absence of phagocytosis.

Williams A, COLE P Br J Exp Pathol. 1981; 62(1):1-7.

PMID: 6261780 PMC: 2041649.

References

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