Characterization of Concatemeric Plasmids of Neisseria Gonorrhoeae

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 May 1

PMID 6404838

Citations 7

Authors

S R Johnson

B E Anderson

J W Biddle

G H Perkins

W E DeWitt

Affiliations

Soon will be listed here.

Abstract

Three strains of Neisseria gonorrhoeae carried novel plasmids of 7.8 megadaltons (mdal) molecular mass in addition to plasmids previously observed in this organism. The presence of the 7.8-mdal plasmids was not accompanied by any distinguishable phenotype in the strain possessing them. Analysis of plasmid DNA with restriction endonucleases showed that these plasmids were composed of three directly repeated copies of a 2.6-mdal cryptic plasmid frequently found in N. gonorrhoeae. In addition, the 7.8-mdal plasmids exhibited characteristics common to the 2.6-mdal plasmid, structural lability and sites resistant to cleavage with HpaII. The concatemeric forms of the cryptic plasmid appear to be stable in these strains and do not undergo internal recombination to produce the 2.6-mdal monomer, nor were higher concatemers detected.

Citing Articles

Four different integrative recombination events involved in the mobilization of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in Escherichia coli.

Scharbaai-Vazquez R, Gonzalez-Caraballo A, Torres-Bauza L Plasmid. 2008; 60(3):200-11.

PMID: 18778732 PMC: 2615557. DOI: 10.1016/j.plasmid.2008.07.004.

Evaluation of plasmids in tetracycline resistant strains of Neisseria gonorrhoeae and Ureaplasma urealyticum in a case of severe urethritis.

Jahn G, Bialasiewicz A, Blenk H Eur J Epidemiol. 1985; 1(4):294-300.

PMID: 3939493 DOI: 10.1007/BF00237105.

Identification of penicillinase producing Neisseria gonorrhoeae in Chile during clinical and microbiological study of gonococcal susceptibility to antimicrobial agents.

Garcia Moreno J, Dillon J, Arroyave R, Maldonado A, Fich F, Salvo A Genitourin Med. 1987; 63(1):6-12.

PMID: 3102348 PMC: 1193997. DOI: 10.1136/sti.63.1.6.

Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid.

Biswas G, Graves J, Schwalbe R, Sparling P J Bacteriol. 1986; 167(2):685-94.

PMID: 3090021 PMC: 212944. DOI: 10.1128/jb.167.2.685-694.1986.

Introduction of cloned genes into Neisseria gonorrhoeae.

Stein D Clin Microbiol Rev. 1989; 2 Suppl:S146-9.

PMID: 2497957 PMC: 358092. DOI: 10.1128/CMR.2.Suppl.S146.

References

OCALLAGHAN C, Morris A, Kirby S, Shingler A . Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972; 1(4):283-8. PMC: 444209. DOI: 10.1128/AAC.1.4.283. View

Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View

Foster R, FOSTER G . Electrophoretic comparison of endonuclease-digested plasmids from Neisseria gonorrhoeae. J Bacteriol. 1976; 126(3):1297-304. PMC: 233156. DOI: 10.1128/jb.126.3.1297-1304.1976. View

Meyers J, Sanchez D, ELWELL L, Falkow S . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976; 127(3):1529-37. PMC: 232949. DOI: 10.1128/jb.127.3.1529-1537.1976. View

Roberts M, ELWELL L, Falkow S . Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol. 1977; 131(2):557-63. PMC: 235464. DOI: 10.1128/jb.131.2.557-563.1977. View

SHORT H, Ploscowe V, Weiss J, Young F . Rapid method for auxotyping multiple strains of Neisseria gonorrhoeae. J Clin Microbiol. 1977; 6(3):244-8. PMC: 274747. DOI: 10.1128/jcm.6.3.244-248.1977. View

Sox T, Mohammed W, Blackman E, Biswas G, Sparling P . Conjugative plasmids in Neisseria gonorrhoeae. J Bacteriol. 1978; 134(1):278-86. PMC: 222244. DOI: 10.1128/jb.134.1.278-286.1978. View

Roberts M, Piot P, Falkow S . The ecology of gonococcal plasmids. J Gen Microbiol. 1979; 114(2):491-4. DOI: 10.1099/00221287-114-2-491. View

Davies J, Normark S . A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases in Neisseria gonorrhoeae. Mol Gen Genet. 1980; 177(2):251-60. DOI: 10.1007/BF00267436. View

10.

van Embden J, van Klingeren B, VAN WIJNGAARDEN L . Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: epidemiology and genetic and molecular characterization of their plasmids. Antimicrob Agents Chemother. 1980; 18(5):789-97. PMC: 284093. DOI: 10.1128/AAC.18.5.789. View

11.

Dillon J, Pauze M . Relationship between plasmid content and auxotype in Neisseria gonorrhoeae isolates. Infect Immun. 1981; 33(2):625-8. PMC: 350746. DOI: 10.1128/iai.33.2.625-628.1981. View

12.

Dillon J, Pauze M . Appearance in Canada of Neisseria gonorrhoeae strains with a 3.2 megadalton penicillinase-producing plasmid and a 24.5 megadalton transfer plasmid. Lancet. 1981; 2(8248):700. DOI: 10.1016/s0140-6736(81)91037-0. View

13.

Sandstrom E, Knapp J, Buchanan T . Serology of Neisseria gonorrhoeae: W-antigen serogrouping by coagglutination and protein I serotyping by enzyme-linked immunosorbent assay both detect protein I antigens. Infect Immun. 1982; 35(1):229-39. PMC: 351020. DOI: 10.1128/iai.35.1.229-239.1982. View

14.

Novick R . Micro-iodometric assay for penicillinase. Biochem J. 1962; 83:236-40. PMC: 1243538. DOI: 10.1042/bj0830236. View

15.

Mayer L, Holmes K, Falkow S . Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974; 10(4):712-7. PMC: 423011. DOI: 10.1128/iai.10.4.712-717.1974. View