Inhibition of Metabolic Cooperation in Chinese Hamster V79 Cells by Various Organic Solvents and Simple Compounds

Overview

Journal Cell Biol Toxicol

Publisher Springer

Specialties Cell Biology
Toxicology

Date 1984 Oct 1

PMID 6400921

Citations 7

Authors

T H Chen

T J Kavanagh

C C Chang

J E Trosko

Affiliations

Soon will be listed here.

Abstract

Gap-junctional intercellular communication is a biological process implicated in the regulation of cell proliferation and differentiation. Metabolic cooperation between 6-thioguanine-sensitive and resistant Chinese hamster cells, in vitro, has been used as a means to detect chemicals which can inhibit this form of intercellular communication. To further characterize this in vitro system as a potential screening assay for potential teratogens, tumor promoters and reproductive toxicants, a series of common solvents as well as other chemicals representing eight different functional groups, i.e., alcohols with straight or side chains, glycols, ketones, esters, ethers, phenols, aldehydes, amines and amino compounds and oxygen-heterocyclic compounds, were tested for their ability to inhibit colony-formation and to inhibit metabolic cooperation. A wide range of effects were observed which suggested a structure/activity relationship between a chemical's ability to inhibit gap junction-mediated intercellular communication and the cytotoxicity of a chemical. Possible mechanisms affecting the modulation of gap junctional communication by these chemicals are discussed.

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References

Boutwell R . The function and mechanism of promoters of carcinogenesis. CRC Crit Rev Toxicol. 1974; 2(4):419-43. DOI: 10.3109/10408447309025704. View

Yotti L, Chang C, Trosko J . Elimination of metabolic cooperation in Chinese hamster cells by a tumor promoter. Science. 1979; 206(4422):1089-91. DOI: 10.1126/science.493994. View

Chen T . In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977; 104(2):255-62. DOI: 10.1016/0014-4827(77)90089-1. View

Collins S, Ruscetti F, Gallagher R, Gallo R . Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978; 75(5):2458-62. PMC: 392573. DOI: 10.1073/pnas.75.5.2458. View

Jone C, Trosko J, Chang C, Fujiki H, Sugimura T . Inhibition of intercellular communication in Chinese hamster V79 cells by teleocidin. Gan. 1982; 73(6):874-8. View

Welsch F, Stedman D . Inhibition of metabolic cooperation between Chinese hamster V79 cells by structurally diverse teratogens. Teratog Carcinog Mutagen. 1984; 4(3):285-301. DOI: 10.1002/tcm.1770040304. View

Hooper M . Metabolic co-operation between mammalian cells in culture. Biochim Biophys Acta. 1982; 651(2-3):85-103. DOI: 10.1016/0304-419x(82)90008-7. View

Fitzgerald D, Murray A . A new intercellular communication assay: its use in studies on the mechanism of tumour promotion. Cell Biol Int Rep. 1982; 6(3):235-42. DOI: 10.1016/0309-1651(82)90075-3. View

Loewenstein W . Junctional intercellular communication: the cell-to-cell membrane channel. Physiol Rev. 1981; 61(4):829-913. DOI: 10.1152/physrev.1981.61.4.829. View

10.

Blakley P, Scott Jr W . Determination of the proximate teratogen of the mouse fetal alcohol syndrome. 1. Teratogenicity of ethanol and acetaldehyde. Toxicol Appl Pharmacol. 1984; 72(2):355-63. DOI: 10.1016/0041-008x(84)90320-x. View

11.

Trosko J, Chang C, Medcalf A . Mechanisms of tumor promotion: potential role of intercellular communication. Cancer Invest. 1983; 1(6):511-26. DOI: 10.3109/07357908309020276. View

12.

Loewenstein W . Molecular and morphological aspects of cell-cell communication. Introductory remarks to the symposium. In Vitro. 1980; 16(12):1007-9. DOI: 10.1007/BF02619250. View

13.

Dorman B, Boreiko C . Limiting factors of the V79 cell metabolic cooperation assay for tumor promoters. Carcinogenesis. 1983; 4(7):873-7. DOI: 10.1093/carcin/4.7.873. View

14.

Sawicki W, Mystkowska E . Phorbol ester-mediated modulation of cell proliferation and primary differentiation of mouse preimplantation embryos. Exp Cell Res. 1981; 136(2):455-8. DOI: 10.1016/0014-4827(81)90025-2. View

15.

Niedel J, KUHN L, Vandenbark G . Phorbol diester receptor copurifies with protein kinase C. Proc Natl Acad Sci U S A. 1983; 80(1):36-40. PMC: 393304. DOI: 10.1073/pnas.80.1.36. View

16.

Schanne F, Zucker A, Farber J, Rubin E . Alcohol-dependent liver cell necrosis in vitro: a new model. Science. 1981; 212(4492):338-40. DOI: 10.1126/science.7209533. View

17.

Huber B, Brown N . 12-O-tetradecanoylphorbol-13-acetate actions on macromolecular synthesis, ornithine decarboxylase, and cellular differentiation of the rat embryonic visceral yolk sac in culture. Cancer Res. 1983; 43(11):5552-9. View

18.

Schanne F, Kane A, Young E, Farber J . Calcium dependence of toxic cell death: a final common pathway. Science. 1979; 206(4419):700-2. DOI: 10.1126/science.386513. View

19.

Murray A, Fitzgerald D . Tumor promoters inhibit metabolic cooperation in cocultures of epidermal and 3T3 cells. Biochem Biophys Res Commun. 1979; 91(2):395-401. DOI: 10.1016/0006-291x(79)91535-3. View

20.

Shiba Y . Modulatory action of 12-O-tetradecanoylphorbol-13-acetate on bud production inHydra. Wilehm Roux Arch Dev Biol. 2017; 190(6):305-307. DOI: 10.1007/BF00863266. View