Study of the Biological Effects of Theophylline on V79 Cells: Viability, Membrane Permeability, and Metabolic Cooperation

Overview

Journal Cell Biol Toxicol

Publisher Springer

Specialties Cell Biology
Toxicology

Date 1991 Apr 1

PMID 1716177

Citations 1

Authors

A Gabelova

D Slamenova

T Bohusova

Affiliations

Soon will be listed here.

Abstract

The effect of theophylline, a specific inhibitor of phosphodiesterase, on gap junction-mediated intercellular communication between Chinese hamster V79 cells was examined. It was found that addition of theophylline to coculture of 6-thioguanine-resistant (TGr) and 6-thioguanine-sensitive (TGs) V79 cells significantly increased the recovery of TGr cells. This finding indicates an inhibition of metabolic cooperation of V79 cells by theophylline. Theophylline was tested at concentrations less than 0.3 mg/ml, which were neither cytotoxic (after short or continuous exposure) nor inhibited the synthesis of DNA, RNA, and proteins. At the tested concentrations, no change was found in the membrane permeability of cells. Theophylline did not increase the incorporation of glucose into the cells.

Citing Articles

Cell culture assays for chemicals with tumor-promoting or tumor-inhibiting activity based on the modulation of intercellular communication.

Budunova I, Williams G Cell Biol Toxicol. 1994; 10(2):71-116.

PMID: 7953912 DOI: 10.1007/BF00756491.

References

Houslay M, Dipple I, Gordon L . Phenobarbital selectively modulates the glucagon-stimulated activity of adenylate cyclase by depressing the lipid phase separation occurring in the outer half of the bilayer of liver plasma membranes. Biochem J. 1981; 197(3):675-81. PMC: 1163180. DOI: 10.1042/bj1970675. View

Pascal J, Beranger S, Pinhas H, Poizot A, Desiles J . New antihistaminic theophylline or theobromine derivatives. J Med Chem. 1985; 28(5):647-52. DOI: 10.1021/jm50001a019. View

Walum E, Peterson A . Tritiated 2-deoxy-D-glucose as a probe for cell membrane permeability studies. Anal Biochem. 1982; 120(1):8-11. DOI: 10.1016/0003-2697(82)90310-4. View

Loewenstein W . Junctional intercellular communication: the cell-to-cell membrane channel. Physiol Rev. 1981; 61(4):829-913. DOI: 10.1152/physrev.1981.61.4.829. View

Dolby T, Belmont A, Borun T, Nicolini C . DNA replication, chromatin structure, and histone phosphorylation altered by theophylline in synchronized HeLa S3 cells. J Cell Biol. 1981; 89(1):78-85. PMC: 2111767. DOI: 10.1083/jcb.89.1.78. View

Cheung W . Properties of cyclic 3',5'-nucleotide phosphodiesterase from rat brain. Biochemistry. 1967; 6(4):1079-87. DOI: 10.1021/bi00856a017. View

Pitts J, Finbow M . The gap junction. J Cell Sci Suppl. 1986; 4:239-66. DOI: 10.1242/jcs.1986.supplement_4.15. View

Niedel J, KUHN L, Vandenbark G . Phorbol diester receptor copurifies with protein kinase C. Proc Natl Acad Sci U S A. 1983; 80(1):36-40. PMC: 393304. DOI: 10.1073/pnas.80.1.36. View

Timson J . Theobromine and theophylline. Mutat Res. 1975; 32(2):169-78. DOI: 10.1016/0165-1110(75)90005-6. View

10.

Weinstein D, MAUER I, Katz M, Kazmer S . The effect of methylxanthines on chromosomes of human lyphocytes in culture. Mutat Res. 1975; 31(1):57-61. DOI: 10.1016/0165-1161(75)90064-3. View

11.

Svec J, Svec P, Halcak L, THURZO V . Role of natural prostaglandins in the control of murine mammary tumor virus expression. J Cancer Res Clin Oncol. 1982; 103(1):55-67. DOI: 10.1007/BF00410306. View

12.

Trosko J, Yotti L, Warren S, Tsushimoto G, Chang C . Inhibition of cell-cell communication by tumor promoters. Carcinog Compr Surv. 1982; 7:565-85. View

13.

Hamel E, Yamasaki H, Tayot J . A phorbol ester-binding inhibitory factor from human placenta. Partial purification, characterization and biological effects. IARC Sci Publ. 1984; (56):157-64. View

14.

Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, NISHIZUKA Y . Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982; 257(13):7847-51. View

15.

Kanno Y, Enomoto T, Shiba Y, Yamasaki H . Protective effect of cAMP on tumor promoter-mediated inhibition of cell-cell communication. Exp Cell Res. 1984; 152(1):31-7. DOI: 10.1016/0014-4827(84)90227-1. View

16.

Bell J, Buxton I, Brunton L . Enhancement of adenylate cyclase activity in S49 lymphoma cells by phorbol esters. Putative effect of C kinase on alpha s-GTP-catalytic subunit interaction. J Biol Chem. 1985; 260(5):2625-8. View

17.

Summers S, Walker J, Sando J, Cronin M . Phorbol esters increase adenylate cyclase activity and stability in pituitary membranes. Biochem Biophys Res Commun. 1988; 151(1):16-24. DOI: 10.1016/0006-291x(88)90553-0. View

18.

Striem B, Naim M, Zehavi U, Ronen T . Saccharin induces changes in adenylate cyclase activity in liver and muscle membranes in rats. Life Sci. 1990; 46(11):803-10. DOI: 10.1016/0024-3205(90)90068-3. View

19.

Takeda A, Hashimoto E, Yamamura H, Shimazu T . Phosphorylation of liver gap junction protein by protein kinase C. FEBS Lett. 1987; 210(2):169-72. DOI: 10.1016/0014-5793(87)81330-3. View

20.

NISHIZUKA Y . Perspectives on the role of protein kinase C in stimulus-response coupling. J Natl Cancer Inst. 1986; 76(3):363-70. View