Nucleotide Sequence and Transcriptional Start Point of the Phosphomannose Isomerase Gene (manA) of Escherichia Coli

Overview

Journal Gene

Specialty Molecular Biology

Date 1984 Dec 1

PMID 6397402

Citations 36

Authors

J S Miles

J R Guest

Affiliations

Soon will be listed here.

Abstract

A 1.6-kb MspI-HindIII chromosomal DNA segment, carrying the complete coding region of the 6-phosphomannose isomerase (PMI) structural gene, manA, and the 5' end of the gene encoding the major fumarase activity, fumA, of Escherichia coli K-12, has been sequenced using the chain termination method. The start points of manA and fumA transcripts were located by S1 mapping using 32P-labelled M13 ssDNA probes, and the two genes were shown to be transcribed divergently. The sequence of the 390 amino acid residues comprising the PMI monomer has been deduced, and the predicted Mr of 42 716 agrees with that for the protein of Mr 42 000 identified previously by the maxicell procedure.

Citing Articles

Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.

Sunico V, Piunti I, Bhattacharjee M, Mezzetti B, Caballero J, Munoz-Blanco J Int J Mol Sci. 2024; 25(22).

PMID: 39595971 PMC: 11594270. DOI: 10.3390/ijms252211902.

The discovery of novel noncoding RNAs in 50 bacterial genomes.

Narunsky A, Higgs G, Torres B, Yu D, de Andrade G, Kavita K Nucleic Acids Res. 2024; 52(9):5152-5165.

PMID: 38647067 PMC: 11109978. DOI: 10.1093/nar/gkae248.

Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.

Poles L, Licciardello C, Distefano G, Nicolosi E, Gentile A, La Malfa S Plants (Basel). 2020; 9(8).

PMID: 32722179 PMC: 7465985. DOI: 10.3390/plants9080938.

Elevated vitamin E content improves all-trans β-carotene accumulation and stability in biofortified sorghum.

Che P, Zhao Z, Glassman K, Dolde D, Hu T, Jones T Proc Natl Acad Sci U S A. 2016; 113(39):11040-5.

PMID: 27621466 PMC: 5047201. DOI: 10.1073/pnas.1605689113.

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOG(R)1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens.

Izawati A, Masani M, Ismanizan I, Parveez G Front Plant Sci. 2015; 6:727.

PMID: 26442041 PMC: 4585222. DOI: 10.3389/fpls.2015.00727.