Evaluation on the Effectiveness of 2-deoxyglucose-6-phosphate Phosphatase (DOG(R)1) Gene As a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens

Overview

Journal Front Plant Sci

Date 2015 Oct 7

PMID 26442041

Citations 8

Authors

Abang Masli Dayang Izawati

Mat Yunus Abdul Masani

Ismail Ismanizan

Ghulam Kadir Ahmad Parveez

Affiliations

Soon will be listed here.

Abstract

DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOG(R)1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOG(R)1 gene and 2-DOG for regenerating transgenic oil palm.

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