The Gene and the Pseudogene for Mouse P53 Cellular Tumor Antigen Are Located on Different Chromosomes

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1984 Aug 1

PMID 6387444

Citations 18

Authors

H H Czosnek

B Bienz

D Givol

D D Pravtcheva

F H Ruddle

M Oren

Affiliations

Soon will be listed here.

Abstract

The chromosomal assignments of the two genes encoding the murine p53 cellular tumor antigen were determined by using a panel of mouse-Chinese hamster somatic cell hybrid clones and a mouse p53-specific cDNA clone. One gene, probably the functional member of the family, was found to be on chromosome 11. The other gene, which is probably a processed pseudogene, was assigned to chromosome 14. The potential relevance of these findings to documented cases of chromosome 11 trisomy are also discussed.

Citing Articles

Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening.

Jin W, Deng Y, La Marca J, Lelliott E, Diepstraten S, Konig C Nat Commun. 2025; 16(1):974.

PMID: 39885149 PMC: 11782673. DOI: 10.1038/s41467-025-56282-2.

copy number expansion is associated with the evolution of increased body size and an enhanced DNA damage response in elephants.

Sulak M, Fong L, Mika K, Chigurupati S, Yon L, Mongan N Elife. 2016; 5.

PMID: 27642012 PMC: 5061548. DOI: 10.7554/eLife.11994.

Pseudogene: lessons from PCR bias, identification and resurrection.

Chen S, Ma K, Zeng J Mol Biol Rep. 2010; 38(6):3709-15.

PMID: 21116863 DOI: 10.1007/s11033-010-0485-4.

Stem Cells in Aging: Influence of Ontogenic, Genetic and Environmental Factors.

Yunis E, Zuniga J, Koka P, Husain Z, Romero V, Stern J J Stem Cells. 2008; 1(2):125-147.

PMID: 19030125 PMC: 2585945.

Mouse H2 congenic intervals: analysis and use for mapping.

Jiang P, Hansen T, Shreffler D, Miller R Mamm Genome. 1995; 6(9):586-91.

PMID: 8535063 DOI: 10.1007/BF00352362.

References

NICHOLS E, Ruddle F . A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels. J Histochem Cytochem. 1973; 21(12):1066-81. DOI: 10.1177/21.12.1066. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Croce C, ADEN C, KOPROWSKI H . Tumorigenicity of mouse-human diploid hybrids in nude mice. Science. 1975; 190(4220):1200-2. DOI: 10.1126/science.173020. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

McCormick F, Harlow E . Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells. J Virol. 1980; 34(1):213-24. PMC: 288687. DOI: 10.1128/JVI.34.1.213-224.1980. View

CRAWFORD L, Lane D, Denhardt D, Harlow E, Nicklin P, Osborn K . Characterization of the complex between SV40 large T antigen and the 53K host protein in transformed mouse cells. Cold Spring Harb Symp Quant Biol. 1980; 44 Pt 1,:179-87. DOI: 10.1101/sqb.1980.044.01.021. View

DEustachio P, Bothwell A, Takaro T, Baltimore D, Ruddle F . Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains. J Exp Med. 1981; 153(4):793-800. PMC: 2186131. DOI: 10.1084/jem.153.4.793. View

McCormick F, Clark R, Harlow E, Tjian R . SV40 T antigen binds specifically to a cellular 53 K protein in vitro. Nature. 1981; 292(5818):63-5. DOI: 10.1038/292063a0. View

Campisi J, Medrano E, Morreo G, Pardee A . Restriction point control of cell growth by a labile protein: evidence for increased stability in transformed cells. Proc Natl Acad Sci U S A. 1982; 79(2):436-40. PMC: 345758. DOI: 10.1073/pnas.79.2.436. View

10.

Carmon Y, Czosnek H, Nudel U, Shani M, Yaffe D . DNAase I sensitivity of genes expressed during myogenesis. Nucleic Acids Res. 1982; 10(10):3085-98. PMC: 320692. DOI: 10.1093/nar/10.10.3085. View

11.

Mercer W, Nelson D, DeLeo A, Old L, Baserga R . Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells. Proc Natl Acad Sci U S A. 1982; 79(20):6309-12. PMC: 347110. DOI: 10.1073/pnas.79.20.6309. View

12.

WINCHESTER G . p53 protein and control of growth. Nature. 1983; 303(5919):660-1. DOI: 10.1038/303660a0. View

13.

Chen S, Blanck G, Pollack R . Pre-crisis mouse cells show strain-specific covariation in the amount of 54-kilodalton phosphoprotein and in susceptibility to transformation by simian virus 40. Proc Natl Acad Sci U S A. 1983; 80(18):5670-4. PMC: 384320. DOI: 10.1073/pnas.80.18.5670. View

14.

Shen D, Real F, DeLeo A, Old L, Marks P, Rifkind R . Protein p53 and inducer-mediated erythroleukemia cell commitment to terminal cell division. Proc Natl Acad Sci U S A. 1983; 80(19):5919-22. PMC: 390188. DOI: 10.1073/pnas.80.19.5919. View

15.

Miller D, MILLER O . Chromosomes and cancer in the mouse: studies in tumors, established cell lines, and cell hybrids. Adv Cancer Res. 1983; 39:153-82. DOI: 10.1016/s0065-230x(08)61035-0. View

16.

Oren M, Bienz B, Givol D, Rechavi G, Zakut R . Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen. EMBO J. 1983; 2(10):1633-9. PMC: 555338. DOI: 10.1002/j.1460-2075.1983.tb01637.x. View

17.

Oren M, Bienz B, Lavie V, Hazum S, Givol D . A single gene and a pseudogene for the cellular tumour antigen p53. Nature. 1983; 306(5943):594-7. DOI: 10.1038/306594a0. View

18.

Rotter V, Wolf D, Pravtcheva D, Ruddle F . Chromosomal assignment of the murine gene encoding the transformation-related protein p53. Mol Cell Biol. 1984; 4(2):383-5. PMC: 368708. DOI: 10.1128/mcb.4.2.383-385.1984. View

19.

Czosnek H, Nudel U, Shani M, Barker P, Pravtcheva D, Ruddle F . The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. EMBO J. 1982; 1(11):1299-305. PMC: 553208. DOI: 10.1002/j.1460-2075.1982.tb01314.x. View