Molecular Homogeneity of Heat-stable Enterotoxins Produced by Bovine Enterotoxigenic Escherichia Coli

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1984 Jul 1

PMID 6376355

Citations 4

Authors

A M Saeed

N S Magnuson

N Sriranganathan

D Burger

W Cosand

Affiliations

Soon will be listed here.

Abstract

Heat-stable enterotoxins (STs) from four strains of bovine enterotoxigenic Escherichia coli representing four serogroups were purified to homogeneity by utilizing previously published purification schemata. Biochemical characterization of the purified STs showed that they met the basic criteria for the heat-stable enterotoxins of E. coli. Amino acid analysis of the purified STs revealed that they were peptides of identical amino acid composition. This composition consisted of 18 residues of 10 different amino acids, 6 of which were cysteine. The amino acid composition of the four ST peptides was identical to that reported for the STs of human and porcine E. coli. In addition, complete sequence analysis of two of the ST peptides and partial sequencing of several others revealed strong homology to the sequences of STs from human and porcine E. coli and to the sequence predicted from the last 18 codons of the transposon Tn1681. There was also substantial homology to the sequence predicted from the ST-coding genetic element of human E. coli, which may indicate the existence of identical bioactive configuration among ST peptides of E. coli strains of various host origins. These data support the hypothesis that STs produced by human, bovine, and porcine E. coli are coded by a closely related genetic element which may have originated from a single, widely disseminated transposon.

Citing Articles

Enterotoxigenic Escherichia coli Heat-Stable Toxin Increases the Rate of Zinc Release from Metallothionein and Is a Zinc- and Iron-Binding Peptide.

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PMID: 32238569 PMC: 7113584. DOI: 10.1128/mSphere.00146-20.

Revised amino acid sequence for a heat-stable enterotoxin produced by an Escherichia coli strain (18D) that is pathogenic for humans.

Thompson M, Giannella R Infect Immun. 1985; 47(3):834-6.

PMID: 3882570 PMC: 261404. DOI: 10.1128/iai.47.3.834-836.1985.

Blinded, two-laboratory comparative analysis of Escherichia coli heat-stable enterotoxin production by using monoclonal antibody enzyme-linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes.

Thompson M, Jordan R, Luttrell M, Brandwein H, Kaper J, Levine M J Clin Microbiol. 1986; 24(5):753-8.

PMID: 3533986 PMC: 269023. DOI: 10.1128/jcm.24.5.753-758.1986.

Characterization of heat-stable enterotoxin from a hypertoxigenic Escherichia coli strain that is pathogenic for cattle.

Saeed A, Magnuson N, Gay C, Greenberg R Infect Immun. 1986; 53(2):445-7.

PMID: 3525417 PMC: 260898. DOI: 10.1128/iai.53.2.445-447.1986.

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