Comparative Evaluation of Different Enzyme-linked Immunosorbent Assay Systems for the Detection of Staphylococcal Enterotoxins A, B, C, and D

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1984 Jan 1

PMID 6361061

Citations 29

Authors

H Fey

H Pfister

O Ruegg

Affiliations

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Abstract

We compared four versions of the enzyme-linked immunosorbent assay for their suitability for detecting staphylococcal enterotoxins. The sandwich with labeled antibody proved to be the best. We used it with a sorbent consisting of antibody-coated polystyrene spheres reacted with 20 ml of food extract. The sensitivity of the test was 0.1 ng of enterotoxin per ml, which is far below clinical relevance. The succinimidyl-pyridyl-dithio-propionate enzyme coupling method of Pharmacia was superior to the two-step glutaraldehyde technique. Interfering protein A was eliminated by the simple addition of normal rabbit serum to the extracts. A diagnostic kit is now available.

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