Dk-restricted Antiinfluenza Cytotoxic T-cell Clone Loses One of Its Two Alloreactivities

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1984 Jan 1

PMID 6332067

Citations 1

Authors

D C Wraith

Affiliations

Soon will be listed here.

Abstract

K6, an influenza-specific cytotoxic T (Tc)-cell clone derived from C3H/He mice, was Dk-restricted. Further analysis showed that K6 also recognized Dd and Dq as allotargets. The clonal nature of the three recognition specificities was confirmed by cold-target cell inhibition. After extensive in vitro selection, K6 lost the ability to lyse Dd cells while retaining both other specificities. The role of the Lyt-2 molecule in antigen-specific self-H-2-restricted vs allospecific recognition was investigated by inhibition with the Lyt-2-specific monoclonal antibody 53-6.7. Both activities were proportionately inhibited, indicating that the Lyt-2 molecule is equally important for both recognition specificities of this Tc-cell clone.

Citing Articles

Control of immune interferon release by cytotoxic T-cell clones specific for influenza.

Taylor P, Wraith D, Askonas B Immunology. 1985; 54(4):607-14.

PMID: 2579894 PMC: 1453554.

References

Mullbacher A, Blanden R . Cross-reactivity patterns of murine cytotoxic T lymphocytes. Cell Immunol. 1979; 43(1):70-81. DOI: 10.1016/0008-8749(79)90151-5. View

Finberg R, Burakoff S, CANTOR H, Benacerraf B . Biological significance of alloreactivity: T cells stimulated by Sendai virus-coated syngeneic cells specifically lyse allogeneic target cells. Proc Natl Acad Sci U S A. 1978; 75(10):5145-9. PMC: 336281. DOI: 10.1073/pnas.75.10.5145. View

Ozato K, Sachs D . Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981; 126(1):317-21. View

Wraith D, Holtkamp B, Askonas B . Loss of serological determinants does not affect recognition of H-2Kk target cells by an influenza-specific cytotoxic T cell clone. Eur J Immunol. 1983; 13(9):762-6. DOI: 10.1002/eji.1830130912. View

Heber-Katz E, Schwartz R, Matis L, Hannum C, Fairwell T, Appella E . Contribution of antigen-presenting cell major histocompatibility complex gene products to the specificity of antigen-induced T cell activation. J Exp Med. 1982; 155(4):1086-99. PMC: 2186641. DOI: 10.1084/jem.155.4.1086. View

Mullbacher A, Blanden R, Brenan M . Neonatal tolerance of major histocompatibility complex antigens alters Ir gene control of the cytotoxic T cell response to vaccinia virus. J Exp Med. 1983; 157(4):1324-38. PMC: 2186975. DOI: 10.1084/jem.157.4.1324. View

Braciale T, Andrew M, Braciale V . Simultaneous expression of H-2-restricted and alloreactive recognition by a cloned line of influenza virus-specific cytotoxic T lymphocytes. J Exp Med. 1981; 153(5):1371-6. PMC: 2186158. DOI: 10.1084/jem.153.5.1371. View

Gaston J, Rickinson A, Epstein M . Cross-reactivity of self-HLA-restricted Epstein-Barr virus-specific cytotoxic T lymphocytes for allo-HLA determinants. J Exp Med. 1983; 158(6):1804-21. PMC: 2187177. DOI: 10.1084/jem.158.6.1804. View

Askonas B, Mullbacher A, Ashman R . Cytotoxic T-memory cells in virus infection and the specificity of helper T cells. Immunology. 1982; 45(1):79-84. PMC: 1555148. View

10.

Pfizenmaier K, Jung H, Kurrle R, Rollinghoff M, Wagner H . Anti H-2Dd alloreactivity mediated by herpes-simplex-virus specific cytotoxic H-2k T lymphocytes is associated with H-2Dk. Immunogenetics. 2012; 10(4):395-404. DOI: 10.1007/BF01561589. View

11.

Townsend A, Taylor P, Mellor A, Askonas B . Recognition of Db and Kb gene products by influenza-specific cytotoxic T cells. Immunogenetics. 1983; 17(3):283-94. DOI: 10.1007/BF00364412. View

12.

Ben-Nun A, Lando Z, Dorf M, Burakoff S . Analysis of cross-reactive antigen-specific T cell clones. Specific recognition of two major histocompatibility complex (MHC) and two non-MHC antigens by a single clone. J Exp Med. 1983; 157(6):2147-53. PMC: 2187034. DOI: 10.1084/jem.157.6.2147. View

13.

Lindahl K, Wilson D . Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors. J Exp Med. 1977; 145(3):508-22. PMC: 2180715. DOI: 10.1084/jem.145.3.508. View

14.

Stringfellow M, Wraith D, Askonas B . Cytotoxic T-cell recognition of influenza-infected target cells varies in different H-2k mouse strains. Immunogenetics. 1983; 18(2):177-81. DOI: 10.1007/BF00368548. View

15.

Stukart M, Boes J, Melief C . Mutations in H-2Kb influence the specificity of alloreactive effector cells included in the repertoire of H-2Db-restricted cytotoxic T lymphocytes against Moloney leukemia virus. Immunogenetics. 1983; 17(4):427-36. DOI: 10.1007/BF00372460. View

16.

Gotze D, Nadeau J, Wakeland E, Berry R, Bonhomme F, EGOROV I . Histocompatibility-2 system in wild mice. X. Frequencies of H-2 and Ia antigens in wild mice from Europe and Africa. J Immunol. 1980; 124(6):2675-81. View

17.

Hunig T, Bevan M . Antigen recognition by cloned cytotoxic T lymphocytes follows rules predicted by the altered-self hypothesis. J Exp Med. 1982; 155(1):111-25. PMC: 2186565. DOI: 10.1084/jem.155.1.111. View

18.

von Boehmer H, Hengartner H, Nabholz M, Lernhardt W, SCHREIER M, Haas W . Fine specificity of a continuously growing killer cell clone specific for H-Y antigen. Eur J Immunol. 1979; 9(8):592-7. DOI: 10.1002/eji.1830090804. View

19.

Askonas B, Lin Y . An influenza specific T-killer clone is restricted to H-2Ld and cross-reacts with Dk region. Immunogenetics. 1982; 16(1):83-7. DOI: 10.1007/BF00364444. View

20.

Harris D, MacDonald H, Cerottini J . Antigen recognition by H-2-restricted cytolytic T lymphocytes is not mediated by two independent receptors. J Exp Med. 1984; 159(1):330-5. PMC: 2187206. DOI: 10.1084/jem.159.1.330. View